Identification of adequate vehicles to carry nerve regeneration inducers using tubulisation

BMC Neuroscience

Research Article

Jean de Oliveira Ramos¹  Natália Fernanda Gaspar de Almeida¹  Adriana Helena do Nascimento-Elias¹  Maria Cristina Lopes Schiavoni¹  Bruno César Fresnesdas¹  Wilson Marques Junior¹  Amilton Antunes Barreira²  Ana Paula Santos³ 

[1] Department of Neurosciences, School of Medicine of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil;Department of Neurosciences, School of Medicine of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil;Departamento de Neurociências, Faculdade de Medicina de Ribeirão Preto, CEP: 14049-900, Ribeirão Preto, São Paulo, Av Bandeirantes 3900, Brasil;Federal University of Jequitinhonha and Mucuri Valleys (UFVJM), Diamantina, Minas Gerais, Brazil;

关键词: Pharmaceutical vehicle;    Agarose;    Culture medium;    Collagen;    Nerves;    Peripheral nerves;    Nerve regeneration;    Neuronal plasticity;    Sciatic nerve;    Wallerian degeneration;   

DOI  :  10.1186/1471-2202-13-100

 received in 2012-01-05, accepted in 2012-06-26,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundAxonal regeneration depends on many factors, such as the type of injury and repair, age, distance from the cell body and distance of the denervated muscle, loss of surrounding tissue and the type of injured nerve. Experimental models use tubulisation with a silicone tube to research regenerative factors and substances to induce regeneration. Agarose, collagen and DMEM (Dulbecco’s modified Eagle’s medium) can be used as vehicles. In this study, we compared the ability of these vehicles to induce rat sciatic nerve regeneration with the intent of finding the least active or inert substance. The experiment used 47 female Wistar rats, which were divided into four experimental groups (agarose 4%, agarose 0.4%, collagen, DMEM) and one normal control group. The right sciatic nerve was exposed, and an incision was made that created a 10 mm gap between the distal and proximal stumps. A silicone tube was grafted onto each stump, and the tubes were filled with the respective media. After 70 days, the sciatic nerve was removed. We evaluated the formation of a regeneration cable, nerve fibre growth, and the functional viability of the regenerated fibres.ResultsComparison among the three vehicles showed that 0.4% agarose gels had almost no effect on provoking the regeneration of peripheral nerves and that 4% agarose gels completely prevented fibre growth. The others substances were associated with profuse nerve fibre growth.ConclusionsIn the appropriate concentration, agarose gel may be an important vehicle for testing factors that induce regeneration without interfering with nerve growth.

【 授权许可】

Unknown   
© Nascimento-Elias et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.