Diversity, Phylogeny, anticancer and antimicrobial potential of fungal endophytes associated with Monarda citriodora L.

BMC Microbiology

Research Article

Shagun Vaid¹  Shashank Singh¹  Meenu Katoch²  Shipra Phull² 

[1] Cancer Pharmacology Divison, Indian Institute of Integrative Medicine, Jammu, India;Microbial Biotechnology Division, Indian Institute of Integrative Medicine, Jammu, India;

关键词: Endophytic fungi;    Monarda citriodora;    Antimicrobial activity;    Tube dilution method;    Cytotoxicity;    MTT assay;   

DOI  :  10.1186/s12866-017-0961-2

 received in 2016-06-22, accepted in 2017-02-21,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundPresent study focuses on diversity and distribution analysis of endophytic fungi associated with different tissues of the Monarda citriodora Cerv. ex Lag. (Lamiaceae/Labiatae). Anticancer and antimicrobial potential of isolated endophytes have also been investigated.ResultsA total of twenty eight fungal endophytes belonging to 11 different genera were isolated from this plant. All the endophytic fungi belonged to the Ascomycota phylum. The leaves were immensely rich in fungal species, while roots showed the highest tissue specific fungal dominance. Out of 28 fungal species, 72% endophytic extracts were found cytotoxic against one or more human cancer cell lines. The most prominent anticancer activity (IC50 value <10 μg/mL) was shown by MC-14 L (Fusarium oxysporum), MC-14 F (F. oxysporum), MC-18 L (Aspergillus fumigatus), MC-24 L (Cladosporium tenuissimum), MC-25 L (Fusarium sp.), MC-26 F (F. oxysporum) extracts. 75% of the extracts showed antimicrobial activities in agar disc-diffusion assay and 27% in the tube dilution method (MIC <100 μg/mL) respectively against the tested pathogens. Extracts of MC-14 L (F. oxysporum) and MC-18 L (A. fumigatus) displayed broad spectrum antimicrobial activity.ConclusionsThese results indicated that M. citriodora harbors a rich fungal endophytic community with anticancer and antimicrobial activities. The isolated endophyte MC-24 L (C. tenuissimum) has the potential to be a source of novel cytotoxic/antimicrobial compounds. This is the first report of diversity of fungal endophytes isolated from M. citriodora.

【 授权许可】

CC BY   
© The Author(s). 2017