Construction of a high-density genetic linkage map and QTL mapping of growth and cold tolerance traits in Takifugu fasciatus

BMC Genomics

Research

Tao Wang¹  Ying Zhang¹  Jie Li¹  Shaowu Yin¹  Peng Chu¹  Ruhua Shang¹ 

[1] College of Marine Science and Engineering, Nanjing Normal University, 210023, Nanjing, Jiangsu, China;

关键词: Takifugu fasciatus;    Genetic linkage map;    QTL mapping;    Growth and cold tolerance traits;   

DOI  :  10.1186/s12864-023-09740-4

 received in 2023-07-02, accepted in 2023-10-13,  发布年份 2023

来源: Springer

【 摘 要 】

Takifugu fasciatus is an aquaculture species with high economic value. In recent years, problems such as environmental pollution and inbreeding have caused a serious decline in T. fasciatus germplasm resources. In this study, a high-density genetic linkage map was constructed by whole-genome resequencing. The map consists of 4891 bin markers distributed across 22 linkage groups (LGs), with a total genetic coverage of 2381.353 cM and a mean density of 0.535 cM. Quantitative trait locus (QTL) localization analysis showed that a total of 19 QTLs associated with growth traits of T. fasciatus in the genome-wide significance threshold range, distributed on 11 LGs. In addition, 11 QTLs associated with cold tolerance traits were identified, each scattered on a different LG. Furthermore, we used QTL localization analysis to screen out three candidate genes (IGF1, IGF2, ADGRB) related to growth in T. fasciatus. Meanwhile, we screened three candidate genes (HSP90, HSP70, and HMGB1) related to T. fasciatus cold tolerance. Our study can provide a theoretical basis for the selection and breeding of cold-tolerant or fast-growing T. fasciatus.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

Fig. 4

Fig. 5

Fig. 4

12936_2015_665_Article_IEq1.gif

Fig. 1

Fig. 2

Fig. 4