Construction of a genetic linkage map, QTLs mapping for low salinity and growth-related traits and identification of the candidate genes in Pacific white shrimp (Litopenaeus vannamei)

Aquaculture Reports

Yuping Sun¹  Wen Huang²  Xiaopeng Wu³  Kai Peng⁴  Ping Zhong⁴  Jinshang Liu⁴  Chaozheng Li⁴  Xiaoying Chen⁴  Zhongneng Xu⁴  Hongxia Zhao⁴  Bing Chen⁴  Huo Li⁵ 

[1] Fishery College, Guangdong Ocean University, Zhanjiang 524088, China;Guangdong Provincial Engineering and Technology Research Center, Guangdong Jinyang Biotechnology Co. Ltd., Maoming 525027, China;Institute of Hydrobiology, Jinan University, Guangzhou 510632, China;Fisheries Research Centre, Key Laboratory of Animal Nutrition and Feed Science in South China of Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;Institute of Hydrobiology, Jinan University, Guangzhou 510632, China;

关键词: Litopenaeus vannamei;    Genetic linkage map;    QTL;    Low-salinity stress;    Growth-related traits;   

DOI  :  

来源: DOAJ

【 摘 要 】

Low-salinity tolerance and growth are crucial economic traits of shrimp in areas with low-salinity water. In the current study, an F1 full-sib family of Pacific white shrimp (Litopenaeus vannamei) was obtained by the “semidirectional cross” method, and 2 parents and 146 progenies were genotyped using double-digest restriction site-associated DNA sequencing technology (ddRAD-Seq). A total of 3335 high-quality markers were identified and used to construct a genetic linkage map. This genetic map comprised 48 linkage groups (LGs), and the total map length spanned 3954.462 centimorgan (cM) with an average marker interval of 1.186 cM. Three quantitative trait loci (QTLs) for low-salinity tolerance and 3 QTLs for growth (body weight) were detected under the condition of restricted logarithmic odds (LOD) score ≥ 2.50. With the assistance of the genome of L. vannamei and relevant annotations, 3 potential growth-related genes (sob, TJP1 and ZBTB24) and 3 low-salinity response candidate genes (Rnf168, CNOT2 and RPB3) were identified from the genomic scaffolds. Moreover, the qPCR results showed that the expression levels of the 3 candidate genes were significantly affected by low-salinity stress, indicating that they possibly play an important role in low-salinity stress. Here, we present the first report of mapping of low-salinity tolerance QTLs in the genetic mapping of L. vannamei, which will be beneficial for the selection of shrimp breeding markers and might help promote the study of the L. vannamei genome.

【 授权许可】

Unknown