Real-time PCR assay for discrimination of Plasmodium ovale curtisi and Plasmodium ovale wallikeri in the Ivory Coast and in the Comoros Islands

Malaria Journal

Research

Jérôme Desplans¹  Daniel Parzy¹  Christophe Fraisier¹  Frédérique Bauffe² 

[1] UMR-MD3, Aix-Marseille Université, Institut de recherche Biomédicale des Armées, Marseille, France;UMR-MD3, Aix-Marseille Université, Institut de recherche Biomédicale des Armées, Marseille, France;WHIDIAG, 100 route des houillères, 13590, Meyreuil, France;

关键词: Plasmodium ovale curtisi;    Plasmodium ovale wallikeri;    Real-time TaqMan® PCR;    Diagnostic;    Sympatry;   

DOI  :  10.1186/1475-2875-11-307

 received in 2012-06-11, accepted in 2012-08-18,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundPlasmodium ovale is one of the five malaria species infecting humans. Recent data have shown that the name of this neglected species masks two distinct genotypes also called curtisi and wallikeri. Some authors show that these species could be sympatric. These two subspecies are not differentiated by microscopy techniques and malaria rapid diagnostic tests. This diagnostic defect is the result of low parasitaemia, antigenic polymorphism and absence of antibodies performance and requires the use of sequencing techniques. An accurate and easy discrimination detection method is necessary.MethodsA new molecular assay was developed to easily identify the two genotypes of P. ovale. This tool allowed the study of 90 blood samples containing P. ovale, confirmed by molecular biology techniques, which were obtained from patients with imported malaria.ResultsThe new marker was validated on well genotyped samples. The genotype of 90 P. ovale samples mainly imported from the Ivory Coast and the Comoros Islands was easily and quickly realized. The distribution of the two subspecies was described with a significant number of samples and showed that the two genotypes were present in the studied countries.ConclusionThis work confirms the presence of the two species in the same country for the first time, in the Ivory Coast and the Comoros Islands. A better genotyping of P. ovale types may improve a better characterization of the clinical pathophysiology for each.

【 授权许可】

Unknown   
© Bauffe et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.