Novel protein extraction approach using micro-sized chamber for evaluation of proteins eluted from formalin-fixed paraffin-embedded tissue sections

Proteome Science

Methodology

Shun-ichiro Ogura¹  Yutaka Aoki²  Taka-Aki Sato²  Kanako Wakabayashi-Nakao³  Tohru Mochizuki³  Keiichi Hatakeyama³  Isamu Hayashi⁴  Takashi Nakajima⁴  Ken Yamaguchi⁵ 

[1] Frontier Research Center, Tokyo Institute of Technology, 4259-B102 Nagatsuta-cho, 226-8051, Midori-ku, Yokohama, Japan;Life Science Research Center, Shimadzu Corporation, 1 Nishinokyo-Kuwabara-cho, 604-8511, Nakagyo-ku, Kyoto, Japan;Medical Genetics Division, Shizuoka Cancer Center Research Institute, 1007 Shimonagakubo, Nagaizumi-cho, 411-8777, Sunto-gun, Shizuoka, Japan;Pathology Division, Shizuoka Cancer Center Hospital, 1007 Shimonagakubo, Nagaizumi-cho, 411-8777, Sunto-gun, Shizuoka, Japan;Shizuoka Cancer Center Hospital and Research Institute, 1007 Shimonagakubo, Nagaizumi-cho, 411-8777, Sunto-gun, Shizuoka, Japan;

关键词: Antigen retrieval;    Colon adenoma;    Colon cancer;    FFPE tissue;    Gastric cancer;    Heat-induced antigen retrieval;    Mass spectrometry;    Micro-sized chamber;   

DOI  :  10.1186/1477-5956-10-19

 received in 2012-02-16, accepted in 2012-03-23,  发布年份 2012

来源: Springer

【 摘 要 】

We describe a novel antigen-retrieval method using a micro-sized chamber for mass spectrometry (MS) analysis to identify proteins that are preferentially eluted from formalin-fixed paraffin-embedded (FFPE) samples. This approach revealed that heat-induced antigen retrieval (HIAR) from an FFPE sample fixed on a glass slide not only improves protein identification, but also facilitates preferential elution of protein subsets corresponding to the properties of antigen-retrieval buffers. Our approach may contribute to an understanding of the mechanism of HIAR.

【 授权许可】

CC BY   
© Hatakeyama et al; licensee BioMed Central Ltd. 2012