Novel protein extraction approach using micro-sized chamber for evaluation of proteins eluted from formalin-fixed paraffin-embedded tissue sections

Proteome Science

关键词: Antigen retrieval;    Colon adenoma;    Colon cancer;    FFPE tissue;    Gastric cancer;    Heat-induced antigen retrieval;    Mass spectrometry;    Micro-sized chamber;   

DOI  :  10.1186/1477-5956-10-19

来源: DOAJ

【 摘 要 】

Abstract

We describe a novel antigen-retrieval method using a micro-sized chamber for mass spectrometry (MS) analysis to identify proteins that are preferentially eluted from formalin-fixed paraffin-embedded (FFPE) samples. This approach revealed that heat-induced antigen retrieval (HIAR) from an FFPE sample fixed on a glass slide not only improves protein identification, but also facilitates preferential elution of protein subsets corresponding to the properties of antigen-retrieval buffers. Our approach may contribute to an understanding of the mechanism of HIAR.

【 授权许可】

Unknown