High prevalence of antibodies against polyomavirus WU, polyomavirus KI, and human bocavirus in German blood donors

BMC Infectious Diseases

Research Article

Andreas Opitz¹  Benedikt Weissbrich²  Christiane Prifert²  Moritz Ewald²  Jörg Schubert²  Barbara Scheiner²  Florian Neske² 

[1] Institute of Transfusion Medicine and Hemotherapy, University Clinic Würzburg, Josef-Schneider-Str. 2, 97080, Würzburg, Germany;Institute of Virology and Immunobiology, University of Würzburg, Versbacher Str. 7, 97078, Würzburg, Germany;

关键词: Blood Donor;    Seropositivity Rate;    Recombinant Baculoviruses;    Nasopharyngeal Aspirate;    Human Bocavirus;   

DOI  :  10.1186/1471-2334-10-215

 received in 2009-06-28, accepted in 2010-07-20,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundDNA of the polyomaviruses WU (WUPyV) and KI (KIPyV) and of human bocavirus (HBoV) has been detected with varying frequency in respiratory tract samples of children. However, only little is known about the humoral immune response against these viruses. Our aim was to establish virus-specific serological assays and to determine the prevalence of immunoglobulin G (IgG) against these three viruses in the general population.MethodsThe capsid proteins VP1 of WUPyV and KIPyV and VP2 of HBoV were cloned into baculovirus vectors and expressed in Sf9 insect cells. IgG antibodies against WUPyV VP1, KIPyV VP1, and HBoV VP2 were determined by immunofluorescence assays in 100 plasma samples of blood donors.ResultsThe median age of the blood donors was 31 years (range 20 - 66 yrs), 52% were male. 89% of the samples were positive for WUPyV IgG (median age 31 yrs, 49.4% male), 67% were positive for KIPyV IgG (median age 32 yrs, 46.3% male), and 76% were positive for HBoV IgG (median age 32 yrs, 51.3% male). For WUPyV and HBoV, there were no significant differences of the seropositivity rates with respect to age groups or gender. For KIPyV, the seropositivity rate increased significantly from 59% in the age group 20 - 29 years to 100% in the age group > 50 years.ConclusionsHigh prevalences of antibodies against WUPyV, KIPyV, and HBoV were found in plasma samples of healthy adults. The results indicate that primary infection with these viruses occurs during childhood or youth. For KIPyV, the seropositivity appears to increase further during adulthood.

【 授权许可】

Unknown   
© Neske et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.