Improved detection of genus-specific Alphavirus using a generic TaqMan® assay

BMC Microbiology

Methodology Article

Claude Giry¹  Ghislaine Li-Pat-Yuen¹  Marie-Christine Jaffar-Bandjee²  Bénédicte Roquebert³  Philippe Gasque⁴ 

[1] Centre National Arbovirus Associé, CHU de la Réunion-Site Nord, Saint-Denis, La Réunion, France;Laboratoire de microbiologie, CHU de la Réunion-Site Nord, Saint-Denis, La Réunion, France;Centre National Arbovirus Associé, CHU de la Réunion-Site Nord, Saint-Denis, La Réunion, France;Laboratoire de microbiologie, CHU de la Réunion-Site Nord, Saint-Denis, La Réunion, France;Laboratoire d’immunologie clinique et expérimentale ZOI (LICE-OI), CHU de la Réunion-Site Nord, Saint-Denis, La Réunion, France;UMR PIMIT, Processus Infectieux en Milieu Insulaire Tropical, Plateforme Technologique CYROI, Sainte-Clotilde, La Réunion, France;Centre National Arbovirus Associé, CHU de la Réunion-Site Nord, Saint-Denis, La Réunion, France;Laboratoire de microbiologie, CHU de la Réunion-Site Nord, Saint-Denis, La Réunion, France;UMR PIMIT, Processus Infectieux en Milieu Insulaire Tropical, Plateforme Technologique CYROI, Sainte-Clotilde, La Réunion, France;Laboratoire d’immunologie clinique et expérimentale ZOI (LICE-OI), CHU de la Réunion-Site Nord, Saint-Denis, La Réunion, France;UMR PIMIT, Processus Infectieux en Milieu Insulaire Tropical, Plateforme Technologique CYROI, Sainte-Clotilde, La Réunion, France;

关键词: Alphavirus;    Molecular diagnosis;    Virus emergence;   

DOI  :  10.1186/s12866-017-1080-9

 received in 2017-02-01, accepted in 2017-07-18,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundAlphaviruses are arthropod borne RNA viruses of medical importance. Geographical expansion of mosquitoes of the Aedes genus in the past decades has been associated with major Alphavirus-associated outbreaks. Climate changes and intensification of air travels have favored vector expansion and virus dissemination in new territories leading to virus emergence not only in tropical areas but also in temperate regions. The detection of emergence is based upon surveillance networks with epidemiological and laboratory investigation.MethodA specific, sensitive and rapid screening test for genus-specific Alphavirus is critically required. To address this issue, we developed a new molecular assay targeting nsP4 gene and using a TaqMan® real time RT-PCR method for the specific detection of all major Alphavirus genus members.ResultsThis assay was tested for specificity using several Alphavirus species. We also tested successfully clinical sensitivity using patient’s samples collected during the Chikungunya outbreak of 2005–2006 in the Indian Ocean.ConclusionsThis new pan-Alphavirus molecular diagnostic tool offers great potential for exclusion diagnosis and emergence detection given its broad specificity restricted to Alphavirus genus.

【 授权许可】

CC BY   
© The Author(s). 2017