Sindbis virus induced phosphorylation of IRF3 in human embryonic kidney cells is not dependent on mTOR:

Innate Immunity

Nisha RDhanushkodi¹ 

关键词: Alphavirus;    PI3K/Akt/mTOR;    IRF3;    ISG56;    IP-10;    Rapamycin;    torin1;   

DOI  :  10.1177/1753425911406944

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

The mammalian target of rapamycin (mTOR) plays critical roles in immunity. We previously showed that infection of human embryonic kidney (HEK) cells with Sindbis virus (SIN), an enveloped RNA alphavirus, profoundly suppresses Akt/mTOR signaling, and host translation late during infection. To understand how SIN mediated suppression of mTOR affects innate response, we analyzed phosphorylation of interferon regulatory factor 3 (IRF3) and expression of two antiviral genes. Here we show strong phosphorylation of IRF3, and an increase in mRNA levels for antiviral genes interferon stimulated gene (ISG)56 and interferon gamma inducible protein (IP)-10 when intracellular viral RNA levels are high during late infection. The mTOR inhibitors rapamycin and torin1 do not block, but mildly upregulate these responses. Even after prolonged treatment with Ly294002, the PI3K inhibitor only partially blocks SIN induced phosphorylation of IRF3. While Ly294002 treatment downregulated the SIN induced expression of ISG56 mRNA le...

【 授权许可】

CC BY