Does mosquito mass-rearing produce an inferior mosquito?

Malaria Journal

Research

Hanano Yamada¹  Adel B. Ali¹  Jeremie R. L. Gilles¹  Wadaka Mamai²  Abdoulaye Diabaté³  Roch K. Dabiré³  Hamidou Maïga⁴  Nanwintoun S. Bimbile-Somda⁴  Dieudonné D. Soma⁵  Florence Fournet⁶  Rosemary S. Lees⁷  Georges A. Ouédraogo⁸  Nelius Venter⁹ 

[1] Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna, Austria;Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna, Austria;Institut de Recherche Agricole pour le Développement (IRAD), Yaoundé, Cameroon;Institut de Recherche en Sciences de la Santé/Centre Muraz, BP 545, Bobo-Dioulasso, Burkina Faso;Institut de Recherche en Sciences de la Santé/Centre Muraz, BP 545, Bobo-Dioulasso, Burkina Faso;Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna, Austria;Institut de Recherche en Sciences de la Santé/Centre Muraz, BP 545, Bobo-Dioulasso, Burkina Faso;Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna, Austria;Université Nazi Boni, Bobo-Dioulasso, Burkina Faso;Institut de Recherche pour le Développement (IRD), MIVEGEC, BP 64501, 34394, Montpellier Cedex 5, France;Liverpool Insect Testing Establishment (LITE), Vector Biology Department, Liverpool School of Tropical Medicine, Pembroke Place, L3 5QA, Liverpool, UK;Université Nazi Boni, Bobo-Dioulasso, Burkina Faso;Vector Control Reference Laboratory, Centre for Opportunistic, Tropical & Hospital Infections, National Institute for Communicable Diseases / Wits Research Institute for Malaria, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa;

关键词: Anopheles arabiensis;    Sterile insect technique;    Mass-rearing;    Competitiveness;   

DOI  :  10.1186/s12936-017-2012-8

 received in 2017-02-02, accepted in 2017-09-04,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThe success of the sterile insect technique depends, among other things, on continuous releases of sexually competitive sterile males within the target area. Several factors (including high rearing density and physical manipulation, such as larvae and pupae separation) can influence the quality of males produced in mass-rearing facilities. The different steps in mass production in the laboratory may modify the behaviour of mosquitoes, directly or through loss of natural characters as a result of adaptation to lab rearing, and lead to the competitiveness of sterile male being reduced. In the present study, the objective was to evaluate the effect of mass-rearing conditions on sterile male sexual competitiveness in semi-field cages compared to routine small scale laboratory rearing methods.MethodsAnopheles arabiensis immature stages were reared both on a large scale using a rack and tray system developed by the FAO/IAEA (MRS), and on a small scale using standard laboratory rearing trays (SRS). Mosquito life history traits such as pupation rate, emergence rate, adult size as well as the effect of irradiation on adult longevity were evaluated. Moreover, 5–6 day old mosquitoes were released into field cages and left for two nights to mate and the mating competitiveness between sterile mass-reared males and fertile males reared on a small scale when competing for small scale reared virgin females was investigated. Resulting fertility in a treatment ratio of 1:1:1 (100 irradiated males: 100 non-irradiated males: 100 virgin females) was compared to control cages with 0:100:100 (non-irradiated control) and 100:0:100 (irradiated control).ResultsNo significant differences in life history parameters were observed between rearing methods. The competitiveness index of mass reared males (0.58) was similar to males reared on a small scale (0.59). A residual fertility rate of 20% was observed in the irradiated control (100:0:100), measured as the percentage of eggs collected from the cages which developed to adulthood. No significant difference was observed (t = 0.2896, df = 4, P = 0.7865) between the rearing treatments (MRS and SRS) in the fertility rate, a measure of mating competitiveness.ConclusionsThe results showed that the FAO/IAEA mass-rearing process did not affect mosquito life history parameters or the mating competitiveness of males.

【 授权许可】

CC BY   
© The Author(s) 2017