Two differentially structured collagen scaffolds for potential urinary bladder augmentation: proof of concept study in a Göttingen minipig model

Journal of Translational Medicine

Research

Joachim O. Grosse¹  Katja Stollenwerk¹  Isabella M. Zraik¹  Dorothea Leonhäuser¹  Volker Seifarth²  Pramod K. Srinivasan³  Rene H. Tolba³  Michael Vogt⁴ 

[1] Department of Urology, RWTH Aachen University Hospital, Pauwelsstraße 30, 52074, Aachen, Germany;Department of Urology, RWTH Aachen University Hospital, Pauwelsstraße 30, 52074, Aachen, Germany;FB 9 Department of Biomedical Engineering, Laboratory of Medical and Molecular Biology, Aachen University of Applied Sciences, Jülich, Germany;Institute for Laboratory Animal Science and Experimental Surgery, RWTH Aachen University Hospital, Aachen, Germany;Interdisciplinary Center for Clinical Research IZKF Aachen, RWTH Aachen University Hospital, Aachen, Germany;

关键词: Tissue engineering;    Urinary bladder;    Collagen scaffold;    Autologous cell seeding;    Large animal model;   

DOI  :  10.1186/s12967-016-1112-5

 received in 2016-11-08, accepted in 2016-12-18,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThe repair of urinary bladder tissue is a necessity for tissue loss due to cancer, trauma, or congenital abnormalities. Use of intestinal tissue is still the gold standard in the urological clinic, which leads to new problems and dysfunctions like mucus production, stone formation, and finally malignancies. Therefore, the use of artificial, biologically derived materials is a promising step towards the augmentation of this specialised tissue. The aim of this study was to investigate potential bladder wall repair by two collagen scaffold prototypes, OptiMaix 2D and 3D, naïve and seeded with autologous vesical cells, as potential bladder wall substitute material in a large animal model.MethodsSix Göttingen minipigs underwent cystoplastic surgery for tissue biopsy and cell isolation followed by implantation of unseeded scaffolds. Six weeks after the first operation, scaffolds seeded with the tissue cultured autologous urothelial and detrusor smooth muscle cells were implanted into the bladder together with additional unseeded scaffolds for comparison. Cystography and bladder ultrasound were performed to demonstrate structural integrity and as leakage test of the implantation sites. Eighteen, 22, and 32 weeks after the first operation, two minipigs respectively were sacrificed and the urinary tract was examined via different (immunohistochemical) staining procedures and the usage of two-photon laser scanning microscopy.ResultsBoth collagen scaffold prototypes in vivo had good ingrowth capacity into the bladder wall including a quick lining with urothelial cells. The ingrowth of detrusor muscle tissue, along with the degradation of the scaffolds, could also be observed throughout the study period.ConclusionsWe could show that the investigated collagen scaffolds OptiMaix 2D and 3D are a potential material for bladder wall substitution. The material has good biocompatible properties, shows a good cell growth of autologous cells in vitro, and a good integration into the present bladder tissue in vivo.

【 授权许可】

CC BY   
© The Author(s) 2017