Clonal diversity of the glutamate dehydrogenase gene in Giardia duodenalisfrom Thai Isolates: evidence of genetic exchange or Mixed Infections?

BMC Microbiology

Research Article

Peerapan Tan-ariya¹  Suradej Siripattanapipong¹  Wilai Saksirisampant²  Saovanee Leelayoova³  Mathirut Mungthin³  Parima Boontanom³  RC Andrew Thompson⁴ 

[1] Department of Microbiology, Faculty of Science, Mahidol University, Rama VI Rd, 10400, Bangkok, Thailand;Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Rama IV Rd, 10330, Bangkok, Thailand;Department of Parasitology, Phramongkutklao College of Medicine, Rajawithi Rd, 10400, Bangkok, Thailand;WHO Collaborating Centre for the Molecular Epidemiology of Parasitic Infections, School of Veterinary and Biomedical Science, Murdoch University, South Street, 6150, Murdoch, Western Australia, Australia;

关键词: Giardia duodenalis;    glutamate dehydrogenase;    genetic diversity;    genetic exchange;   

DOI  :  10.1186/1471-2180-11-206

 received in 2011-03-23, accepted in 2011-09-20,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundThe glutamate dehydrogenase gene (gdh) is one of the most popular and useful genetic markers for the genotypic analysis of Giardia duodenalis (syn. G. lamblia, G. intestinalis), the protozoan that widely causes enteric disease in humans. To determine the distribution of genotypes of G. duodenalis in Thai populations and to investigate the extent of sequence variation at this locus, 42 fecal samples were collected from 3 regions of Thailand i.e., Central, Northern, and Eastern regions. All specimens were analyzed using PCR-based genotyping and recombinant subcloning methods.ResultsThe results showed that the prevalence of assemblages A and B among these populations was approximately equal, 20 (47.6%) and 22 (52.4%), respectively. Sequence analysis revealed that the nucleotide diversity of assemblage B was significantly greater than that in assemblage A. Among all assemblage B positive specimens, the allelic sequence divergence within isolates was detected. Nine isolates showed mixed alleles, ranged from three to nine distinct alleles per isolate. Statistical analysis demonstrated the occurrence of genetic recombination within subassemblages BIII and BIV was likely.ConclusionThis study supports increasing evidence that G. duodenalis has the potential for genetic exchange.

【 授权许可】

Unknown   
© Siripattanapipong et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.