Clonal diversity of the glutamate dehydrogenase gene in Giardia duodenalis from Thai Isolates: evidence of genetic exchange or Mixed Infections?

BMC Microbiology

Peerapan Tan-ariya²  Wilai Saksirisampant³  Parima Boontanom⁴  RC Andrew Thompson¹  Mathirut Mungthin⁴  Saovanee Leelayoova⁴  Suradej Siripattanapipong² 

[1] WHO Collaborating Centre for the Molecular Epidemiology of Parasitic Infections, School of Veterinary and Biomedical Sciences, Murdoch University, South Street, Murdoch, Western Australia, 6150, Australia;Department of Microbiology, Faculty of Science, Mahidol University, Rama VI Rd., Bangkok, 10400, Thailand;Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Rama IV Rd., Bangkok, 10330, Thailand;Department of Parasitology, Phramongkutklao College of Medicine, Rajawithi Rd., Bangkok, 10400, Thailand

关键词: genetic exchange;    genetic diversity;    glutamate dehydrogenase;    Giardia duodenalis;   

Others  :  1222214
DOI  :  10.1186/1471-2180-11-206

 received in 2011-03-23, accepted in 2011-09-20,  发布年份 2011

【 摘 要 】

Background

The glutamate dehydrogenase gene (gdh) is one of the most popular and useful genetic markers for the genotypic analysis of Giardia duodenalis (syn. G. lamblia, G. intestinalis), the protozoan that widely causes enteric disease in humans. To determine the distribution of genotypes of G. duodenalis in Thai populations and to investigate the extent of sequence variation at this locus, 42 fecal samples were collected from 3 regions of Thailand i.e., Central, Northern, and Eastern regions. All specimens were analyzed using PCR-based genotyping and recombinant subcloning methods.

Results

The results showed that the prevalence of assemblages A and B among these populations was approximately equal, 20 (47.6%) and 22 (52.4%), respectively. Sequence analysis revealed that the nucleotide diversity of assemblage B was significantly greater than that in assemblage A. Among all assemblage B positive specimens, the allelic sequence divergence within isolates was detected. Nine isolates showed mixed alleles, ranged from three to nine distinct alleles per isolate. Statistical analysis demonstrated the occurrence of genetic recombination within subassemblages BIII and BIV was likely.

Conclusion

This study supports increasing evidence that G. duodenalis has the potential for genetic exchange.

【 授权许可】

   
2011 Siripattanapipong et al; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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