Activation of transcription factor AP-1 in response to thermal injury in rat small intestine and IEC-6 cells

BMC Gastroenterology

Research Article

Tao Liu¹  Linshu Jiang¹  Zhimin Gao¹  Hong Zhao¹  Fenghua Liu¹  Yonghong Zhang¹  Xiaolin Hou¹  Xiaoxi Liu²  Changrong Wan³ 

[1] Beijing Key Laboratory for Dairy Cow Nutrition, Beijing University of Agriculture, No. 7, Beinong Road, Changping District, 102206, Beijing, P. R. China;Beijing Key Laboratory for Dairy Cow Nutrition, Beijing University of Agriculture, No. 7, Beinong Road, Changping District, 102206, Beijing, P. R. China;TCVM Laboratory, College of Veterinary Medicine, China Agricultural University, 100193, Beijing, China;TCVM Laboratory, College of Veterinary Medicine, China Agricultural University, 100193, Beijing, China;

关键词: AP-1;    NF-κB;    Heat stress;    Rat small intestine;    IEC-6;   

DOI  :  10.1186/s12876-015-0309-z

 received in 2014-08-13, accepted in 2015-06-29,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundOur previous studies indicated that heat stress can cause significant damage to the intestinal epithelium and induce differential expression of many genes in rat small intestine. The transcription factors AP-1 and NF-κB, which act as important mediators by binding to specific DNA sequences within gene promoters, regulate the transcription of genes associated with immune regulation, stress response and cell fate.MethodsTo determine whether AP-1 and NF-κB are involved in hyperthermia-induced injury in rat small intestine and IEC-6 cells, we investigated their activity, and the expression of related proteins, by electrophoretic mobility shift assays and western blotting, respectively.ResultsHeat stress resulted in severe damage to the epithelium of the small intestine. The cell morphology and viability were obviously altered when IEC-6 cell was exposed to hyperthermia. AP-1 was activated in the small intestine of heat-stressed rats, as was phosphorylation of the JNK signaling pathway. In IEC-6 cell line, AP-1 activation in groups exposed to 42 °C for 1 h, 2 h and 4 h was significantly increased. In contrast, NF-κB was not activated in both in vivo and in vitro models.ConclusionThese results reveal that AP-1 is likely to play an important role in regulating gene transcription in rat small intestine and IEC-6 cells during exposure to heat stress.

【 授权许可】

Unknown   
© Zhang et al. 2015. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.