期刊论文详细信息
BMC Gastroenterology
Activation of transcription factor AP-1 in response to thermal injury in rat small intestine and IEC-6 cells
Fenghua Liu1  Linshu Jiang1  Xiaolin Hou1  Zhimin Gao1  Xiaoxi Liu2  Changrong Wan2  Tao Liu1  Hong Zhao1  Yonghong Zhang1 
[1] Beijing Key Laboratory for Dairy Cow Nutrition, Beijing University of Agriculture, No. 7, Beinong Road, Changping District, Beijing 102206, P. R. China;TCVM Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
关键词: IEC-6;    Rat small intestine;    Heat stress;    NF-κB;    AP-1;   
Others  :  1224274
DOI  :  10.1186/s12876-015-0309-z
 received in 2014-08-13, accepted in 2015-06-29,  发布年份 2015
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【 摘 要 】

Background

Our previous studies indicated that heat stress can cause significant damage to the intestinal epithelium and induce differential expression of many genes in rat small intestine. The transcription factors AP-1 and NF-κB, which act as important mediators by binding to specific DNA sequences within gene promoters, regulate the transcription of genes associated with immune regulation, stress response and cell fate.

Methods

To determine whether AP-1 and NF-κB are involved in hyperthermia-induced injury in rat small intestine and IEC-6 cells, we investigated their activity, and the expression of related proteins, by electrophoretic mobility shift assays and western blotting, respectively.

Results

Heat stress resulted in severe damage to the epithelium of the small intestine. The cell morphology and viability were obviously altered when IEC-6 cell was exposed to hyperthermia. AP-1 was activated in the small intestine of heat-stressed rats, as was phosphorylation of the JNK signaling pathway. In IEC-6 cell line, AP-1 activation in groups exposed to 42 °C for 1 h, 2 h and 4 h was significantly increased. In contrast, NF-κB was not activated in both in vivo and in vitro models.

Conclusion

These results reveal that AP-1 is likely to play an important role in regulating gene transcription in rat small intestine and IEC-6 cells during exposure to heat stress.

【 授权许可】

   
2015 Zhang et al.

【 预 览 】
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