Genome-wide identification and characterization of SnRK2 gene family in cotton (Gossypium hirsutum L.)

BMC Genetics

Research Article

Xiaoyang Ge¹  Xueyan Zhang¹  Eryong Chen¹  Chaojun Zhang¹  Zhao Liu¹  Fuguang Li¹  Zuoren Yang¹  Ji Liu¹  Ge Zhao¹ 

[1] State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, 455000, Anyang, China;

关键词: SnRK2 kinase family;    Upland cotton;    Phylogenetic analysis;    Expression analysis;   

DOI  :  10.1186/s12863-017-0517-3

 received in 2017-01-17, accepted in 2017-05-17,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundSucrose non-fermenting-1-related protein kinase 2 (SnRK2) is a plant-specific serine/threonine kinase family involved in the abscisic acid (ABA) signaling pathway and responds to osmotic stress. A genome-wide analysis of this protein family has been conducted previously in some plant species, but little is known about SnRK2 genes in upland cotton (Gossypium hirsutum L.). The recent release of the G. hirsutum genome sequence provides an opportunity to identify and characterize the SnRK2 kinase family in upland cotton.ResultsWe identified 20 putative SnRK2 sequences in the G. hirsutum genome, designated as GhSnRK2.1 to GhSnRK2.20. All of the sequences encoded hydrophilic proteins. Phylogenetic analysis showed that the GhSnRK2 genes were classifiable into three groups. The chromosomal location and phylogenetic analysis of the cotton SnRK2 genes indicated that segmental duplication likely contributed to the diversification and evolution of the genes. The gene structure and motif composition of the cotton SnRK2 genes were analyzed. Nine exons were conserved in length among all members of the GhSnRK2 family. Although the C-terminus was divergent, seven conserved motifs were present. All GhSnRK2s genes showed expression patterns under abiotic stress based on transcriptome data. The expression profiles of five selected genes were verified in various tissues by quantitative real-time RT-PCR (qRT-PCR). Transcript levels of some family members were up-regulated in response to drought, salinity or ABA treatments, consistent with potential roles in response to abiotic stress.ConclusionsThis study is the first comprehensive analysis of SnRK2 genes in upland cotton. Our results provide the fundamental information for the functional dissection of GhSnRK2s and vital availability for the improvement of plant stress tolerance using GhSnRK2s.

【 授权许可】

CC BY   
© The Author(s). 2017