SNPchiMp: a database to disentangle the SNPchip jungle in bovine livestock

BMC Genomics

Database

Ali Pirani¹  Fiona Brew²  Ezequiel Luis Nicolazzi³  Francesco Strozzi³  Matteo Picciolini³  Alessandra Stella⁴  Cindy Lawley⁵  Robert David Schnabel⁶ 

[1] Affymetrix Inc, 3420 Central Expressway, 95051, Santa Clara, CA, USA;Affymetrix UK Ltd, Mercury Park, Wycombe Lane, HP10 0HH, High Wycombe, UK;Fondazione Parco Tecnologico Padano, Via Einstein, Loc. Cascina Codazza, 26900, Lodi, Italy;Fondazione Parco Tecnologico Padano, Via Einstein, Loc. Cascina Codazza, 26900, Lodi, Italy;Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, Via Einstein, Cascina Codazza, 26900, Lodi, Italy;Illumina Inc, 5200 Illumina Way, 92121, San Diego, CA, USA;University of Missouri, 65203, Columbia, MO, USA;

关键词: SNP chip;    SNP data;    Relational database;    Assembly;    Imputation;    GWAS;    Bovine livestock;   

DOI  :  10.1186/1471-2164-15-123

 received in 2013-09-03, accepted in 2014-02-06,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundCurrently, six commercial whole-genome SNP chips are available for cattle genotyping, produced by two different genotyping platforms. Technical issues need to be addressed to combine data that originates from the different platforms, or different versions of the same array generated by the manufacturer. For example: i) genome coordinates for SNPs may refer to different genome assemblies; ii) reference genome sequences are updated over time changing the positions, or even removing sequences which contain SNPs; iii) not all commercial SNP ID’s are searchable within public databases; iv) SNPs can be coded using different formats and referencing different strands (e.g. A/B or A/C/T/G alleles, referencing forward/reverse, top/bottom or plus/minus strand); v) Due to new information being discovered, higher density chips do not necessarily include all the SNPs present in the lower density chips; and, vi) SNP IDs may not be consistent across chips and platforms. Most researchers and breed associations manage SNP data in real-time and thus require tools to standardise data in a user-friendly manner.DescriptionHere we present SNPchiMp, a MySQL database linked to an open access web-based interface. Features of this interface include, but are not limited to, the following functions: 1) referencing the SNP mapping information to the latest genome assembly, 2) extraction of information contained in dbSNP for SNPs present in all commercially available bovine chips, and 3) identification of SNPs in common between two or more bovine chips (e.g. for SNP imputation from lower to higher density). In addition, SNPchiMp can retrieve this information on subsets of SNPs, accessing such data either via physical position on a supported assembly, or by a list of SNP IDs, rs or ss identifiers.ConclusionsThis tool combines many different sources of information, that otherwise are time consuming to obtain and difficult to integrate. The SNPchiMp not only provides the information in a user-friendly format, but also enables researchers to perform a large number of operations with a few clicks of the mouse. This significantly reduces the time needed to execute the large number of operations required to manage SNP data.

【 授权许可】

CC BY   
© Nicolazzi et al.; licensee BioMed Central Ltd. 2014