Pharmacokinetic analysis of topical tobramycin in equine tears by automated immunoassay

BMC Veterinary Research

Research Article

Brian Skorobohach¹  Andrew W Lyon²  Sarah L Czerwinski³  Renaud Léguillette³ 

[1] Calgary Animal Referral and Emergency Centre, Calgary, AB, Canada;Department of Pathology and Laboratory Medicine, Faculty of Medicine, University of Calgary, Calgary, AB, Canada;Calgary Laboratory Services, Calgary, AB, Canada;Department of Veterinary Clinical and Diagnostic Sciences, Faculty of Veterinary Medicine, University of Calgary, 3330 Hospital Drive NW, T2N 4N1, Calgary, AB, Canada;

关键词: Antibiotic;    Equine;    Pharmacokinetics;    Tear;    Tobramycin;    Topical;    Ophthalmology;   

DOI  :  10.1186/1746-6148-8-141

 received in 2012-03-28, accepted in 2012-08-14,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundOphthalmic antibiotic therapy in large animals is often used empirically because of the lack of pharmacokinetics studies. The purpose of the study was to determine the pharmacokinetics of topical tobramycin 0.3% ophthalmic solution in the tears of normal horses using an automated immunoassay analysis.ResultsThe mean tobramycin concentrations in the tears at 5, 10, 15, 30 minutes and 1, 2, 4, 6 hours after administration were 759 (±414), 489 (±237), 346 (±227), 147 (±264), 27.6 (±28.4), 14.8 (±66.6), 6.7 (±18.6), and 23.4 (±73.4) mg/L. Mean tobramycin concentration was maintained above the MIC90 for commonly isolated bacteria for 68.5 min.ConclusionA single dose of topical tobramycin resulted in therapeutic concentrations of tobramycin in the tears for 1 h after administration. Therapeutic levels of tobramycin remained in equine tears 6 times longer than was reported in rabbit tears.

【 授权许可】

Unknown   
© Czerwinski et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.