Contaminants from dredged sediments alter the transcriptome of Manila clam and induce shifts in microbiota composition

BMC Biology

Research Article

Valerio Matozzo¹  Maria Ciscato¹  Jacopo Fabrello¹  Davide Asnicar²  Andrea Quagliariello³  Giulia Dalla Rovere³  Maria Elena Martino³  Luca Bargelloni³  Luca Peruzza³  Ilaria Bernardini³  Silvia Iori³  Tomaso Patarnello⁴  Massimo Milan⁴  Antonio Marcomini⁵  Elisa Giubilato⁵  Martina Cecchetto⁵  Elena Semenzin⁵  Cinzia Bettiol⁵  Fabiana Corami⁶  Claudio Carrer⁷ 

[1] Department of Biology, University of Padova, Via U. Bassi 58/B, 35131, Padua, Italy;Department of Biology, University of Padova, Via U. Bassi 58/B, 35131, Padua, Italy;Aquatic Bioscience, Huntsman Marine Science Centre, 1 Lower Campus Road, E5B 2L7, St Andrews, New Brunswick, Canada;Department of Comparative Biomedicine and Food Science, University of Padova, Viale Dell’Università 16, Agripolis, 35020, Legnaro, PD, Italy;Department of Comparative Biomedicine and Food Science, University of Padova, Viale Dell’Università 16, Agripolis, 35020, Legnaro, PD, Italy;NFBC, National Future Biodiversity Center, Palermo, Italy;Department of Environmental Sciences, Informatics, and Statistics, Ca’ Foscari University of Venice, Via Torino, 155, 30172, Venice-Mestre, Italy;Department of Environmental Sciences, Informatics, and Statistics, Ca’ Foscari University of Venice, Via Torino, 155, 30172, Venice-Mestre, Italy;Institute of Polar Sciences, CNR-ISP, Foscari University of Venice, Campus Scientifico - CaVia Torino, 155, 30172, Venice-Mestre, Italy;Thetis S.P.a. C/o laboratorio del Provveditorato Interregionale Alle Opere Pubbliche Per Il Veneto, Il Trentino Alto Adige E Il Friuli Venezia Giulia, Venice-Mestre, Italy;

关键词: Manila clam;    Dredged sediments;    Venice lagoon;    Molecular changes;    Microbiota;    Lagoon contamination;   

DOI  :  10.1186/s12915-023-01741-9

 received in 2023-09-01, accepted in 2023-10-17,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundThe reuse of dredged sediments in ports and lagoons is a big issue as it should not affect the quality and the equilibrium of ecosystems. In the lagoon of Venice, sediment management is of crucial importance as sediments are often utilized to built-up structures necessary to limit erosion. However, the impact of sediment reuse on organisms inhabiting this delicate area is poorly known. The Manila clam is a filter-feeding species of high economic and ecological value for the Venice lagoon experiencing a drastic decline in the last decades. In order to define the molecular mechanisms behind sediment toxicity, we exposed clams to sediments sampled from different sites within one of the Venice lagoon navigable canals close to the industrial area. Moreover, we investigated the impacts of dredged sediments on clam’s microbial communities.ResultsConcentrations of the trace elements and organic chemicals showed increasing concentrations from the city of Venice to sites close to the industrial area of Porto Marghera, where PCDD/Fs and PCBs concentrations were up to 120 times higher than the southern lagoon. While bioaccumulation of organic contaminants of industrial origin reflected sediments’ chemical concentrations, metal bioaccumulation was not consistent with metal concentrations measured in sediments probably due to the activation of ABC transporters. At the transcriptional level, we found a persistent activation of the mTORC1 signalling pathway, which is central in the coordination of cellular responses to chemical stress. Microbiota characterization showed the over-representation of potential opportunistic pathogens following exposure to the most contaminated sediments, leading to host immune response activation. Despite the limited acquisition of new microbial species from sediments, the latter play an important role in shaping Manila clam microbial communities.ConclusionsSediment management in the Venice lagoon will increase in the next years to maintain and create new canals as well as to allow the operation of the new mobile gates at the three Venice lagoon inlets. Our data reveal important transcriptional and microbial changes of Manila clams after exposure to sediments, therefore reuse of dredged sediments represents a potential risk for the conservation of this species and possibly for other organisms inhabiting the Venice lagoon.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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