Profiling microRNAs in lung tissue from pigs infected with Actinobacillus pleuropneumoniae

BMC Genomics

Research Article

Mads Bak¹  Niels Tommerup¹  Susanna Cirera²  Agnieszka Podolska³  Merete Fredholm⁴  Christian Anthon⁴  Jan Gorodkin⁴  Kerstin Skovgaard⁵  Peter MH Heegaard⁵ 

[1] Center for non-coding RNA in Technology and Health, University of Copenhagen, Copenhagen, Denmark;Department of Cellular and Molecular Medicine, Panum Institute, Copenhagen, Denmark;Department of Veterinary Clinical and Animal Sciences, Section of Anatomy, Cell Biology, Genetics and Bioinformatics, University of Copenhagen, Faculty of Health and Medical Sciences, Copenhagen, Denmark;Department of Veterinary Clinical and Animal Sciences, Section of Anatomy, Cell Biology, Genetics and Bioinformatics, University of Copenhagen, Faculty of Health and Medical Sciences, Copenhagen, Denmark;Biotech Research & Innovation Centre (BRIC), University of Copenhagen, Copenhagen, Denmark;Department of Veterinary Clinical and Animal Sciences, Section of Anatomy, Cell Biology, Genetics and Bioinformatics, University of Copenhagen, Faculty of Health and Medical Sciences, Copenhagen, Denmark;Center for non-coding RNA in Technology and Health, University of Copenhagen, Copenhagen, Denmark;Innate Immunology Group, National Veterinary Institute, Technical University of Denmark, Copenhagen, Denmark;

关键词: MicroRNA;    RNAseq;    High throughput sequencing;    RT-qPCR;    Actinobacillus pleuropneumoniae;    Pig;    Pleuropneumonia;   

DOI  :  10.1186/1471-2164-13-459

 received in 2012-01-16, accepted in 2012-08-29,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundMicroRNAs (miRNAs) are a class of non-protein-coding genes that play a crucial regulatory role in mammalian development and disease. Whereas a large number of miRNAs have been annotated at the structural level during the latest years, functional annotation is sparse. Actinobacillus pleuropneumoniae (APP) causes serious lung infections in pigs. Severe damage to the lungs, in many cases deadly, is caused by toxins released by the bacterium and to some degree by host mediated tissue damage. However, understanding of the role of microRNAs in the course of this infectious disease in porcine is still very limited.ResultsIn this study, the RNA extracted from visually unaffected and necrotic tissue from pigs infected with Actinobacillus pleuropneumoniae was subjected to small RNA deep sequencing. We identified 169 conserved and 11 candidate novel microRNAs in the pig. Of these, 17 were significantly up-regulated in the necrotic sample and 12 were down-regulated. The expression analysis of a number of candidates revealed microRNAs of potential importance in the innate immune response. MiR-155, a known key player in inflammation, was found expressed in both samples. Moreover, miR-664-5p, miR-451 and miR-15a appear as very promising candidates for microRNAs involved in response to pathogen infection.ConclusionsThis is the first study revealing significant differences in composition and expression profiles of miRNAs in lungs infected with a bacterial pathogen. Our results extend annotation of microRNA in pig and provide insight into the role of a number of microRNAs in regulation of bacteria induced immune and inflammatory response in porcine lung.

【 授权许可】

Unknown   
© Podolska et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.