Leaves play a central role in the adaptation of nitrogen and sulfur metabolism to ammonium nutrition in oilseed rape (Brassica napus)

BMC Plant Biology

Research Article

Pedro M. Aparicio-Tejo¹  Iraide Bejarano²  Marlon de la Peña²  Jon Rodríguez-Escalante²  M. Begoña González-Moro²  Inmaculada Coleto²  Daniel Marino³  Gaëtan Glauser⁴ 

[1] Departamento de Ciencias del Medio Natural, Universidad Pública de Navarra, Pamplona, Navarre, Spain;Department of Plant Biology and Ecology, University of the Basque Country (UPV/EHU), Apdo. 644, E-48080, Bilbao, Spain;Department of Plant Biology and Ecology, University of the Basque Country (UPV/EHU), Apdo. 644, E-48080, Bilbao, Spain;Ikerbasque, Basque Foundation for Science, E-48011, Bilbao, Spain;Neuchâtel Platform of Analytical Chemistry, University of Neuchâtel, Avenue de Bellevaux 51, 2000, Neuchâtel, Switzerland;

关键词: Ammonium;    Brassica napus;    Glucosinolates;    Nitrate;    Nitrogen;    Oilseed rape;    Sulfur;   

DOI  :  10.1186/s12870-017-1100-9

 received in 2017-05-26, accepted in 2017-09-05,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThe coordination between nitrogen (N) and sulfur (S) assimilation is required to suitably provide plants with organic compounds essential for their development and growth. The N source induces the adaptation of many metabolic processes in plants; however, there is scarce information about the influence that it may exert on the functioning of S metabolism. The aim of this work was to provide an overview of N and S metabolism in oilseed rape (Brassica napus) when exposed to different N sources. To do so, plants were grown in hydroponic conditions with nitrate or ammonium as N source at two concentrations (0.5 and 1 mM).ResultsMetabolic changes mainly occurred in leaves, where ammonium caused the up-regulation of enzymes involved in the primary assimilation of N and a general increase in the concentration of N-compounds (NH4+, amino acids and proteins). Similarly, the activity of key enzymes of primary S assimilation and the content of S-compounds (glutathione and glucosinolates) were also higher in leaves of ammonium-fed plants. Interestingly, sulfate level was lower in leaves of ammonium-fed plants, which was accompanied by the down-regulation of SULTR1 transporters gene expression.ConclusionsThe results highlight the impact of the N source on different steps of N and S metabolism in oilseed rape, notably inducing N and S assimilation in leaves, and put forward the potential of N source management to modulate the synthesis of compounds with biotechnological interest, such as glucosinolates.

【 授权许可】

CC BY   
© The Author(s). 2017