Leaves play a central role in the adaptation of nitrogen and sulfur metabolism to ammonium nutrition in oilseed rape (Brassica napus)

BMC Plant Biology

卷:17

Pedro M. Aparicio-Tejo¹  Iraide Bejarano²  Jon Rodríguez-Escalante²  Inmaculada Coleto²  M. Begoña González-Moro²  Daniel Marino²  Marlon de la Peña²  Gaëtan Glauser³ 

[1] Departamento de Ciencias del Medio Natural, Universidad Pública de Navarra;

[2] Department of Plant Biology and Ecology, University of the Basque Country (UPV/EHU);

[3] Neuchâtel Platform of Analytical Chemistry, University of Neuchâtel;

关键词: Ammonium;    Brassica napus;    Glucosinolates;    Nitrate;    Nitrogen;    Oilseed rape;   

DOI  :  10.1186/s12870-017-1100-9

来源: DOAJ

【 摘 要 】

Abstract Background The coordination between nitrogen (N) and sulfur (S) assimilation is required to suitably provide plants with organic compounds essential for their development and growth. The N source induces the adaptation of many metabolic processes in plants; however, there is scarce information about the influence that it may exert on the functioning of S metabolism. The aim of this work was to provide an overview of N and S metabolism in oilseed rape (Brassica napus) when exposed to different N sources. To do so, plants were grown in hydroponic conditions with nitrate or ammonium as N source at two concentrations (0.5 and 1 mM). Results Metabolic changes mainly occurred in leaves, where ammonium caused the up-regulation of enzymes involved in the primary assimilation of N and a general increase in the concentration of N-compounds (NH4 +, amino acids and proteins). Similarly, the activity of key enzymes of primary S assimilation and the content of S-compounds (glutathione and glucosinolates) were also higher in leaves of ammonium-fed plants. Interestingly, sulfate level was lower in leaves of ammonium-fed plants, which was accompanied by the down-regulation of SULTR1 transporters gene expression. Conclusions The results highlight the impact of the N source on different steps of N and S metabolism in oilseed rape, notably inducing N and S assimilation in leaves, and put forward the potential of N source management to modulate the synthesis of compounds with biotechnological interest, such as glucosinolates.

【 授权许可】

Unknown