Vaspin inhibits cytokine-induced nuclear factor-kappa B activation and adhesion molecule expression via AMP-activated protein kinase activation in vascular endothelial cells

Cardiovascular Diabetology

Original Investigation

Yoo La Lee¹  Hae Kyeong Yoon¹  Sang-Wook Kang²  Min Jung Lee³  Joong-Yeol Park³  Yu Mi Kang³  Woo Je Lee³  Chang Hee Jung³ 

[1] Asan Institute of Life Sciences, University of Ulsan College of Medicine, Seoul, Korea;Department of Biomedical Sciences, University of Ulsan College of Medicine, Seoul, Korea;Department of Internal Medicine, University of Ulsan College of Medicine, Poongnap-dong, 138-736, Songpa-gu, Seoul, Korea;

关键词: Vaspin;    Endothelial cells;    AMPK;    NF-κB;    Adhesion molecules;   

DOI  :  10.1186/1475-2840-13-41

 received in 2013-11-20, accepted in 2014-02-11,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundVaspin is an adipocytokine that was recently identified in the visceral adipose tissue of diabetic rats and has anti-diabetic and anti-atherogenic effects. We hypothesized that vaspin prevents inflammatory cytokine-induced nuclear factor-kappa B (NF-κB) activation by activating AMP-activated protein kinase (AMPK) in vascular endothelial cells.MethodsWe examined the effects of vaspin on NF-κB activation and the expression of the NF-κB-mediated genes intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), E-selectin, and monocyte chemoattractant protein-1 (MCP-1). Human aortic endothelial cells (HAECS) were used. Tumor necrosis factor alpha (TNFα) was used as a representative proinflammatory cytokine.ResultsTreatment with vaspin significantly increased the phosphorylation of AMPK and acetyl-CoA carboxylase, the down-stream target of AMPK. Furthermore, treatment with vaspin significantly decreased TNFα-induced activation of NF-κB, as well as the expression of the adhesion molecules ICAM-1, VCAM-1, E-selectin, and MCP-1. These effects were abolished following transfection of AMPKα1-specific small interfering RNA. In an adhesion assay using THP-1 cells, vaspin reduced TNFα-induced adhesion of monocytes to HAECS in an AMPK-dependent manner.ConclusionsVaspin might attenuate the cytokine-induced expression of adhesion molecule genes by inhibiting NF-κB following AMPK activation.

【 授权许可】

Unknown   
© Jung et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.