The role of reactive oxygen intermediates in experimental coccidioidomycois in mice

BMC Microbiology

Research Article

David A Margolis¹  Suganya Viriyakosol²  Theo N Kirkland³  Joshua Fierer³ 

[1] Veterans Affairs San Diego Healthcare System (111F), 3350 La Jolla Village Dr, 92161, San Diego, CA, USA;Department of Medicine, University of California, San Diego, 92093, San Diego, California, USA;GlaxoSmithKline Infectious Diseases Medicine Development Center 5 Moore Drive Research Triangle Park, 27709, NC, USA;Veterans Affairs San Diego Healthcare System (111F), 3350 La Jolla Village Dr, 92161, San Diego, CA, USA;Veterans Medical Research Foundation, 3350 La Jolla Village Dr., 92161, San Diego, CA, USA;Department of Pathology University of California, San Diego, 92093, San Diego, California, USA;Veterans Affairs San Diego Healthcare System (111F), 3350 La Jolla Village Dr, 92161, San Diego, CA, USA;Veterans Medical Research Foundation, 3350 La Jolla Village Dr., 92161, San Diego, CA, USA;Department of Pathology University of California, San Diego, 92093, San Diego, California, USA;Department of Medicine, University of California, San Diego, 92093, San Diego, California, USA;

关键词: Coccidioidomycosis;    host response;    oxidative burst;   

DOI  :  10.1186/1471-2180-11-71

 received in 2010-12-22, accepted in 2011-04-11,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundCoccidioidomycosis is usually a self-limited infection in immunocompentent people. In immunocompentent human beings second infections due to Coccidioides are very rare, indicating that recovery from infection results in protective immunity. In experimental animals, immunization with several different proteins or attenuated mutants protects against a virulent challenge. To explore what mechanisms are responsible for protective immunity, we investigated the course of Coccidioides infection in the gp91phox knock out mouse that has a defect in the oxidative burst that results in chronic granulomatous disease.ResultsWe found that the gp91phox knock out mice were somewhat more resistant to intraperitoneal infection and equally as resistant to low dose intranasal infection, but slightly more susceptible to high dose intranasal infection compared to control mice. The gp91phox knock out mice made a more robust inflammatory response to infection than controls, as measured by histology and production of inflammatory cytokines. The gp91phox knock out mice were as protected by immunization with the recombinant Coccidioides protein Ag2/PRA as the controls were against either intraperitoneal or intranasal infection. Coccidioides immitis arthroconidia and spherules were significantly more resistant to H2O2 treatment in vitro than Aspergillus fumigatus spores.ConclusionThese data suggest that oxidative burst may not be required for protective immunity to coccidioidomycois.

【 授权许可】

Unknown   
© Margolis et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.