The role of reactive oxygen intermediates in experimental coccidioidomycois in mice

BMC Microbiology

Theo N Kirkland¹  Joshua Fierer¹  Suganya Viriyakosol²  David A Margolis³ 

[1] Department of Medicine, University of California, San Diego, San Diego, California, 92093 USA;Department of Pathology University of California, San Diego, San Diego, California, 92093, USA;GlaxoSmithKline Infectious Diseases Medicine Development Center 5 Moore Drive Research Triangle Park, NC 27709 USA

关键词: oxidative burst;    host response;    Coccidioidomycosis;   

Others  :  1225266
DOI  :  10.1186/1471-2180-11-71

 received in 2010-12-22, accepted in 2011-04-11,  发布年份 2011

【 摘 要 】

Background

Coccidioidomycosis is usually a self-limited infection in immunocompentent people. In immunocompentent human beings second infections due to Coccidioides are very rare, indicating that recovery from infection results in protective immunity. In experimental animals, immunization with several different proteins or attenuated mutants protects against a virulent challenge. To explore what mechanisms are responsible for protective immunity, we investigated the course of Coccidioides infection in the gp91^phox knock out mouse that has a defect in the oxidative burst that results in chronic granulomatous disease.

Results

We found that the gp91^phox knock out mice were somewhat more resistant to intraperitoneal infection and equally as resistant to low dose intranasal infection, but slightly more susceptible to high dose intranasal infection compared to control mice. The gp91^phox knock out mice made a more robust inflammatory response to infection than controls, as measured by histology and production of inflammatory cytokines. The gp91^phox knock out mice were as protected by immunization with the recombinant Coccidioides protein Ag2/PRA as the controls were against either intraperitoneal or intranasal infection. Coccidioides immitis arthroconidia and spherules were significantly more resistant to H₂O₂ treatment in vitro than Aspergillus fumigatus spores.

Conclusion

These data suggest that oxidative burst may not be required for protective immunity to coccidioidomycois.

【 授权许可】

   
2011 Margolis et al; licensee BioMed Central Ltd.

【 图 表 】

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