Rapid assessment of myocardial infarct size in rodents using multi-slice inversion recovery late gadolinium enhancement CMR at 9.4T

Journal of Cardiovascular Magnetic Resonance

Technical Notes

Derek J Hausenloy¹  Shiang Y Lim¹  Derek M Yellon¹  King K Cheung²  Mark F Lythgoe²  Anthony N Price² 

[1] The Hatter Cardiovascular Institute, University College London Hospital and Medical School, London, UK;UCL Centre for Advanced Biomedical Imaging, Department of Medicine and UCL Institute of Child Health, University College London, UK;

关键词: Cardiovascular Magnetic Resonance;    Left Anterior Descend;    Late Gadolinium Enhancement;    Infarct Volume;    Late Gadolinium Enhancement Image;   

DOI  :  10.1186/1532-429X-13-44

 received in 2011-04-12, accepted in 2011-09-05,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundMyocardial infarction (MI) can be readily assessed using late gadolinium enhancement (LGE) cardiovascular magnetic resonance (CMR). Inversion recovery (IR) sequences provide the highest contrast between enhanced infarct areas and healthy myocardium. Applying such methods to small animals is challenging due to rapid respiratory and cardiac rates relative to T1 relaxation.MethodsHere we present a fast and robust protocol for assessing LGE in small animals using a multi-slice IR gradient echo sequence for efficient assessment of LGE. An additional Look-Locker sequence was used to assess the optimum inversion point on an individual basis and to determine most appropriate gating points for both rat and mouse. The technique was applied to two preclinical scenarios: i) an acute (2 hour) reperfused model of MI in rats and ii) mice 2 days following non-reperfused MI.ResultsLGE images from all animals revealed clear areas of enhancement allowing for easy volume segmentation. Typical inversion times required to null healthy myocardium in rats were between 300-450 ms equivalent to 2-3 R-waves and ~330 ms in mice, typically 3 R-waves following inversion. Data from rats was also validated against triphenyltetrazolium chloride staining and revealed close agreement for infarct size.ConclusionThe LGE protocol presented provides a reliable method for acquiring images of high contrast and quality without excessive scan times, enabling higher throughput in experimental studies requiring reliable assessment of MI.

【 授权许可】

CC BY   
© Price et al; licensee BioMed Central Ltd. 2011