Quantitation of cell-free DNA and RNA in plasma during tumor progression in rats

Molecular Cancer

Research

Damián García-Olmo¹  Ana I Asensio²  María G Picazo²  Inmaculada Toboso²  Dolores C García-Olmo² 

[1] Department of Surgery, Universidad Autónoma de Madrid and La Paz University Hospital, Madrid, IdiPaz, Spain;Experimental Research Unit, General University Hospital of Albacete, C/ Hermanos Falcó 37, 02006, Albacete, Spain;

关键词: Cell-free nucleic acids;    Plasma;    Quantitation;    Tumor size;    Metastasis;   

DOI  :  10.1186/1476-4598-12-8

 received in 2012-11-13, accepted in 2013-02-01,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundTo clarify the implications of cell-free nucleic acids (cfNA) in the plasma in neoplastic disease, it is necessary to determine the kinetics of their release into the circulation.MethodsTo quantify non-tumor and tumor DNA and RNA in the plasma of tumor-bearing rats and to correlate such levels with tumor progression, we injected DHD/K12-PROb colon cancer cells subcutaneously into syngenic BD-IX rats. Rats were sacrificed and their plasma was analyzed from the first to the eleventh week after inoculation.ResultsThe release of large amounts of non-tumor DNA into plasma was related to tumor development from its early stages. Tumor-specific DNA was detected in 33% of tumor-bearing rats, starting from the first week after inoculation and at an increasing frequency thereafter. Animals that were positive for tumor DNA in the plasma had larger tumors than those that were negative (p = 0.0006). However, the appearance of both mutated and non-mutated DNA fluctuated with time and levels of both were scattered among individuals in each group. The release of non-tumor mRNA was unaffected by tumor progression and we did not detect mutated RNA sequences in any animals.ConclusionsThe release of normal and tumor cfDNA into plasma appeared to be related to individual-specific factors. The contribution of tumor DNA to the elevated levels of plasma DNA was intermittent. The release of RNA into plasma during cancer progression appeared to be an even more selective and elusive phenomenon than that of DNA.

【 授权许可】

Unknown   
© Garcia-Olmo et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.