Sperm DNA fragmentation and oxidation are independent of malondialdheyde

Reproductive Biology and Endocrinology

Research

Nozha Feki Chakroun¹  Afifa Sellami Ben Hamida¹  Nassira Zribi²  Faiza Fakhfakh²  Fatma Ben Abdallah²  Leila Ammar Keskes³  Jalel Gargouri⁴  Henda Elleuch⁴ 

[1] Laboratory of Histology-Embryology, Sfax Faculty of Medicine, Avenue Magida Boulila, 3028, Sfax, Tunisia;Laboratory of Human Molecular Genetics, Sfax Faculty of Medicine, Avenue Magida Boulila, 3028, Sfax, Tunisia;Laboratory of Human Molecular Genetics, Sfax Faculty of Medicine, Avenue Magida Boulila, 3028, Sfax, Tunisia;Laboratory of Histology-Embryology, Sfax Faculty of Medicine, Avenue Magida Boulila, 3028, Sfax, Tunisia;Regional Center of Blood Transfusion of Sfax, El Ain Road, 3029, Sfax, Tunisia;

关键词: Sperm Cell;    Sperm Motility;    Male Infertility;    Semen Sample;    Semen Parameter;   

DOI  :  10.1186/1477-7827-9-47

 received in 2010-10-22, accepted in 2011-04-14,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundThere is clinical evidence to show that sperm DNA damage could be a marker of sperm quality and extensive data exist on the relationship between DNA damage and male fertility status. Detecting such damage in sperm could provide new elements besides semen parameters in diagnosing male infertility. We aimed to assess sperm DNA fragmentation and oxidation and to study the association between these two markers, routine semen parameters and malondialdehyde formation.MethodsSemen samples from 55 men attending the Histology-Embryology Laboratory of Sfax Faculty of Medicine, Tunisia, for semen investigations were analysed for sperm DNA fragmentation and oxidation using flow cytometry. The Sperm was also assessed spectrophotometrically for malondialdehyde formation.ResultsWithin the studied group, 21 patients were nonasthenozoospermic (sperm motility ≥ 50%) and 34 patients were considered asthenozoospermic (sperm motility < 50%). A positive correlation was found between sperm DNA fragmentation and oxidation (p = 0.01; r = 0.33). We also found a negative correlation between sperm DNA fragmentation and some sperm parameters: total motility (p = 0.001; r = -0.43), rapid progressive motility (type a motility) (p = 0.04; r = -0.27), slow progressive motility (type b motility) (p = 0.03; r = -0.28), and vitality (p < 0.001; r = -0.65). Sperm DNA fragmentation was positively correlated with coiled tail (p = 0.01; r = 0.34). The two parameters that were found to be correlated with oxidative DNA damage were leucocytes concentrations (p = 0.01; r = 0.38) and broken neck (p = 0.02; r = 0.29). Sperm MDA levels were negatively correlated with sperm concentration (p < 0.001; r = -0.57), total motility (p = 0.01; r = -0.35) and type a motility (p = 0.03; r = -0.32); but not correlated with DNA fragmentation and DNA oxidation.ConclusionsOur results support the evidence that oxidative stress plays a key role in inducing DNA damage; but nuclear alterations and malondialdehyde don't seem to be synchronous.

【 授权许可】

CC BY   
© Zribi et al; licensee BioMed Central Ltd. 2011