New insight into circRNAs: characterization, strategies, and biomedical applications

Experimental Hematology & Oncology

Review

Shun-Xin Zhu¹  Ke-Jia Pu¹  Heng-Jing Huang¹  Wen-Tao Wang¹  Xin-Yi Feng¹  Yue-Qin Chen¹ 

[1] MOE Key Laboratory of Gene Function and Regulation, Guangdong Province Key Laboratory of Pharmaceutical Functional Genes, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, 510275, Guangzhou, People’s Republic of China;

关键词: circRNA;    Database;    Strategy;    Therapeutic approaches;    Biomedical applications;   

DOI  :  10.1186/s40164-023-00451-w

 received in 2023-08-16, accepted in 2023-09-23,  发布年份 2023

来源: Springer

【 摘 要 】

Circular RNAs (circRNAs) are a class of covalently closed, endogenous ncRNAs. Most circRNAs are derived from exonic or intronic sequences by precursor RNA back-splicing. Advanced high-throughput RNA sequencing and experimental technologies have enabled the extensive identification and characterization of circRNAs, such as novel types of biogenesis, tissue-specific and cell-specific expression patterns, epigenetic regulation, translation potential, localization and metabolism. Increasing evidence has revealed that circRNAs participate in diverse cellular processes, and their dysregulation is involved in the pathogenesis of various diseases, particularly cancer. In this review, we systematically discuss the characterization of circRNAs, databases, challenges for circRNA discovery, new insight into strategies used in circRNA studies and biomedical applications. Although recent studies have advanced the understanding of circRNAs, advanced knowledge and approaches for circRNA annotation, functional characterization and biomedical applications are continuously needed to provide new insights into circRNAs. The emergence of circRNA-based protein translation strategy will be a promising direction in the field of biomedicine.

【 授权许可】

CC BY   
© YUMED Inc. and BioMed Central Ltd. 2023

【 图 表 】

12951_2016_171_Article_IEq1.gif

Fig. 1

Fig. 4

12936_2023_4742_Article_IEq18.gif

Fig. 4