Rapid reconstitution of CD4 T cells and NK cells protects against CMV-reactivation after allogeneic stem cell transplantation

Journal of Translational Medicine

Research

Ellen Meijer¹  Rob Schuurman²  Ronald Jacobi³  Floortje L. Pietersma³  Nening M. Nanlohy³  Esther D. Quakkelaar³  Leonie Ran³  Sanne Spijkers³  Rogier Gaiser³  Henny Otten³  Ingrid M. M. Schellens³  Dan Koning³  Jurgen Kuball⁴  Suzanne van Dorp⁴  Debbie van Baarle⁵  Julia Drylewicz⁶ 

[1] Department of Haematology, VUMC, Amsterdam, The Netherlands;Department of Virology, Utrecht, The Netherlands;Laboratory of Translational Immunology, Department of Immunology, Utrecht, The Netherlands;Laboratory of Translational Immunology, Department of Immunology, Utrecht, The Netherlands;Department of Haematology, Utrecht, The Netherlands;Laboratory of Translational Immunology, Department of Immunology, Utrecht, The Netherlands;Department of Internal Medicine and Infectious Diseases, University Medical Center Utrecht, Utrecht, The Netherlands;Department of Immune Mechanisms, National Institute for Public Health and the environment (RIVM), Center for Infectious Disease Control, Antonie van leeuwenhoeklaan 9, Bilthoven, The Netherlands;Laboratory of Translational Immunology, Department of Immunology, Utrecht, The Netherlands;Theoretical Biology and Bioinformatics, Department of Biology, Utrecht University, Utrecht, The Netherlands;

关键词: Stem cell transplantation;    CMV;    EBV;    Immune reconstitution;   

DOI  :  10.1186/s12967-016-0988-4

 received in 2016-02-04, accepted in 2016-07-26,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundEpstein-Barr virus and Cytomegalovirus reactivations frequently occur after allogeneic stem cell transplantation (SCT).MethodsHere we investigated the role of immune cell reconstitution in the onset and subsequent severity of EBV- and CMV-reactivation. To this end, 116 patients were prospectively sampled for absolute T cell (CD4 and CD8), B-cell (CD19) and NK-cell (CD16 and CD56) numbers weekly post-SCT during the first 3 months and thereafter monthly until 6 months post-SCT. Viral load was monitored in parallel.ResultsIn contrast to the general belief, we found that early T-cell reconstitution does not play a role in the onset of viral reactivation. CMV reactivation in the first 7 weeks after SCT however resulted in higher absolute CD8+ T-cell numbers 6 months post-SCT in patients with high-level reactivation, many of which were CMV-specific. Interestingly, rapid reconstitution of CD4+ T-cells, as well as NK cells and the presence of donor KIR3DL1, are associated with the absence of CMV-reactivation after SCT, suggestive of a protective role of these cells. In contrast, EBV-reactivations were not affected in any way by the level of immune reconstitution after SCT.ConclusionIn conclusion, these data suggest that CD4+ T-cells and NK cells, rather than CD8+ T-cells, are associated with protection against CMV-reactivation.

【 授权许可】

CC BY   
© The Author(s) 2016