Additive transcriptomic variation associated with reproductive traits suggest local adaptation in a recently settled population of the Pacific oyster, Crassostrea gigas

BMC Genomics

Research Article

Lasse Fast Jensen¹  Virgile Quillen²  Arnaud Huvet²  Pierre Boudry²  Rossana Sussarellu³  Florence Cornette⁴  Sylvie Lapègue⁴  Christophe Lelong⁵  Nicolas Bierne⁶ 

[1] Fisheries and Maritime Museum, DK-6710, Esbjerg V, Denmark;Ifremer, Laboratoire des Sciences de l’Environnement Marin UMR 6539 (UBO/CNRS/IRD/Ifremer), Plouzané, France;Ifremer, Laboratoire des Sciences de l’Environnement Marin UMR 6539 (UBO/CNRS/IRD/Ifremer), Plouzané, France;Present address: Ifremer, Laboratoire d’Ecotoxicologie, Nantes, France;Ifremer, SG2M-LGPMM, Laboratoire de Génétique et Pathologie des Mollusques Marins, 17390, La Tremblade, France;UNICAEN, UMR BOREA MNHN, UPMC, UNICAEN, CNRS-7208, IRD207, F-14032, Caen, France;Université Montpellier 2, Montpellier, France;CNRS - Institut des Sciences de l’Evolution, UMR5554, Station Méditerranéenne de l’Environnement Littoral, Sète, France;

关键词: Crassostrea gigas;    Adaptation;    Invasiveness;    Fertility;    Sex-ratio;    Transcriptome;    Microarray;   

DOI  :  10.1186/s12864-015-1972-8

 received in 2015-04-02, accepted in 2015-10-03,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundOriginating from Northeast Asia, the Pacific oyster Crassostrea gigas has been introduced into a large number of countries for aquaculture purpose. Following introduction, the Pacific oyster has turned into an invasive species in an increasing number of coastal areas, notably recently in Northern Europe.MethodsTo explore potential adaptation of reproductive traits in populations with different histories, we set up a common garden experiment based on the comparison of progenies from two populations of Pacific oyster sampled in France and Denmark and their hybrids. Sex ratio, condition index and microarray gene expression in gonads, were analyzed in each progeny (n = 60).ResultsA female-biased sex-ratio and a higher condition index were observed in the Danish progeny, possibly reflecting an evolutionary reproductive strategy to increase the potential success of natural recruitment in recently settled population. Using multifarious statistical approaches and accounting for sex differences we identified several transcripts differentially expressed between the Danish and French progenies, for which additive genetic basis is suspected (showing intermediate expression levels in hybrids, and therefore additivity). Candidate transcripts included mRNA coding for sperm quality and insulin metabolism, known to be implicated in coordinated control and success of reproduction.ConclusionsObserved differences suggest that adaptation of invasive populations might have occurred during expansion acting on reproductive traits, and in particular on a female-biased sex-ratio, gamete quality and fertility.

【 授权许可】

CC BY   
© Sussarellu et al. 2015