Antifungal bio-coating of endotracheal tube built by overexpressing the MCP1 gene of Saccharomyces boulardii and employing hydrogel as a “house” to antagonize Candida albicans

Biomaterials Research

Research Article

Xinya Hou¹  Xuanyi Wang¹  Xiangwei Lv¹  Xiaolong Mao²  Ziqiang Han²  Hui Zhang²  Jianfeng Qiu³  Yunyun Wei⁴ 

[1] Department of Clinical Medicine, Shandong First Medical University and Shandong Academy of Medical Sciences, 250000, Jinan, China;School of Laboratory Animal & Shandong Laboratory Animal Center, Shandong First Medical University & Shandong Academy of Medical Sciences, 250000, Jinan, Shandong, China;School of Radiology, Shandong First Medical University and Shandong Academy of Medical Sciences, 271000, Tai’an, China;Science and Technology Innovation Center, Shandong First Medical University & Shandong Academy of Medical Sciences, 250000, Jinan, China;School of Radiology, The Second Affiliated Hospital of Shandong First Medical University, 271016, Tai’an, China;School of Radiology, Shandong First Medical University and Shandong Academy of Medical Sciences, 271000, Tai’an, China;

关键词: Medical device coating;    Fungal infection;    Probiotics;    Antagonism;    Hydrogel;   

DOI  :  10.1186/s40824-023-00443-1

 received in 2023-06-21, accepted in 2023-10-01,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundFor some ICU patients, an artificial airway must be established with an endotracheal tube, but Candida albicans can easily adhere to the tube surface and form a biofilm, leading to potentially life threatening fungal infections. Therefore, it is urgent to prevent and reduce C. albicans infections introduced by the endotracheal tube. However, there are few antifungal drugs effective against C. albicans, and each of these drugs may have adverse effects on human cells. Saccharomyces boulardii is regarded as an alternative strategy to inhibit the adhesion of C. albicans, but it is affected by environmental stress. We hypothesized that it is feasible to strengthen the antagonistic ability of S. boulardii via encapsulating and genetically modification.MethodsIn this study, a bioactive material carrying the overexpressed MCP1 gene of Saccharomyces boulardii was constructed based on one-step photo-crosslinking. This material achieved spatial growth control of S. boulardii by encapsulating each S. boulardii cell within a hydrogel pore. The bioactive material was coated on an endotracheal tube and tested for its ability to inhibit the adhesion of C. albicans. Additionally, the material’s antagonistic activity towards C. albicans was evaluated by detecting intracellular Adenosine-triphosphate content, reactive oxygen species level and the activity of antioxidative enzymes. Tissue invasion experiment was executed to further evaluate the anti-adhesion ability of S. boulardii bio-coating.ResultsEncapsulating the overexpression of MCP1 by S. boulardii in hydrogel pores enhanced the viability of probiotics in the presence of high salt and oxidation stress. When used as the coating of an endotracheal tube, the S. boulardii bioactive material efficiently inhibited the adhesion of C. albicans by impairing the activities of superoxide dismutase and catalase and disturbing mitochondrial functions. In vivo, the S. boulardii bioactive material coating displayed good biocompatibility and reduced the host tissue invasion and virulence of C. albicans.ConclusionsThe integration of genetic modification and immobilization model breaks the bottleneck of previous application of microorganisms, and provides a new way to prevent fungal infections introduced by endotracheal tubes.Graphical Abstract

【 授权许可】

CC BY   
© The Korean Society for Biomaterials 2023

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