Occlusal disharmony accelerates the initiation of atherosclerosis in apoE knockout rats

Lipids in Health and Disease

Research

Koichiro Irie¹  Daisuke Ekuni²  Yasumasa Endo²  Toshiki Yoneda²  Kenta Kasuyama²  Shinsuke Mizutani²  Manabu Morita²  Tetsuji Azuma²  Takaaki Tomofuji³ 

[1]Department of Preventive Dentistry and Dental Public Health, Aichi-Gakuin University, Kusumoto-cho, Chikusa-ku, 464-8650, Nagoya, Japan

[2]Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, 700-8558, Okayama, Japan

[3]Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, 700-8558, Okayama, Japan

[4]Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School, 2-5-1 Shikata-cho, Kita-ku, 700-8558, Okayama, Japan

关键词: Apolipoprotein E;    Atherosclerosis;    Occlusion;    Stress;    Toll-like receptor;    Vascular cell Adhesion molecule-1;   

DOI  :  10.1186/1476-511X-13-144

 received in 2014-05-21, accepted in 2014-09-02,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundPsychosocial stress is one of the risk factors for atherosclerosis. As occlusal disharmony induces psychological stress, we hypothesized that psychological stress by occlusal disharmony accelerates atherosclerosis. The aim of this study was to investigate the effects of occlusal disharmony on the initiation of atherosclerosis in apolipoprotein E (apoE) knockout rats.MethodsFourteen male apoE-knockout rats (age; 8 weeks) (Sprague–Dawley strain background) were divided into two groups of seven rats: the occlusal disharmony group and the no treatment (control) group. In the occlusal disharmony group, the maxillary molar cusps were cut off for the 8-week experimental period.ResultsIn the occlusal disharmony group, the percentages of the area of total aortic lumen occupied by plaques and lipid were significantly higher than those in the control group (p < 0.05, t-test). The occlusal disharmony group also showed significantly higher serum levels of very low-density lipoprotein-cholesterol (VLDL) and low-density lipoprotein-cholesterol (LDL), plasma levels of corticosterone (1.9, 1.3 and 1.3 times, respectively), higher aortic protein expression levels of vascular cell adhesion molecule-1 (VCAM1) and intercellular adhesion molecule-1 (ICAM1) (1.5 and 1.4 times, respectively), and higher aortic gene expression of levels of VCAM1 and Toll-like receptor 4 (TLR4) (1.9 and 4.3 times, respectively), as compared to the control group (p < 0.05). However, there were no significant differences in serum levels of oxidized LDL, reactive oxygen metabolites and C-reactive protein between the two groups.ConclusionIn apoE knockout rats, occlusal disharmony may induce VCAM1, ICAM1 and TLR4 expression and accelerate the initiation of atherosclerosis.

【 授权许可】

CC BY   
© Ekuni et al.; licensee BioMed Central Ltd. 2014