Polymorphic patterns of the merozoite surface protein-3β in Korean isolates of Plasmodium vivax

Malaria Journal

Research

Sung-Ung Moon¹  Pyo Yun Cho²  Seong Kyu Ahn²  Tong-Soo Kim²  Byoung-Kuk Na³  Woon-Mok Sohn³  Jung-Mi Kang³  Hye-Lim Ju³  Hyeong-Woo Lee⁴ 

[1] Department of Internal Medicine, Seoul National University Bundang Hospital, 463-707, Seongnam, Republic of Korea;Department of Parasitology and Inha Research Institute for Medical Sciences, Inha University School of Medicine, 400-712, Incheon, Republic of Korea;Department of Parasitology and Tropical Medicine, and Institute of Health Sciences, Gyeongsang National University School of Medicine, 660-751, Jinju, Republic of Korea;Department of Pathology, Immunology, and Laboratory Medicine, College of Medicine, University of Florida, J-566, 1275 Center Drive, 32610, Gainesville, FL, USA;

关键词: Plasmodium vivax;    Merozoite surface protein-3β;    Genetic polymorphism;    Natural selection;    Korea;   

DOI  :  10.1186/1475-2875-13-104

 received in 2014-01-02, accepted in 2014-03-07,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundThe merozoite surface protein-3β of Plasmodium vivax (PvMSP-3β) is one of the candidate antigens for blood stage malaria vaccine development. The polymorphisms in PvMSP-3β have been reported in certain P. vivax isolates. However, the diversity of PvMSP-3β throughout its global distribution has not been well understood. In this study, the genetic diversity and the effects of natural selection in PvMSP-3β among P. vivax Korean isolates were analysed.MethodsBlood samples were collected from 95 patients with vivax malaria in Korea. The region flanking full-length PvMSP-3β was amplified by polymerase chain reaction and cloned into a TA cloning vector. The PvMSP-3β sequence of each isolate was determined and the polymorphic characteristics and effects of natural selection were analysed using the DNASTAR, MEGA4, and DnaSP programs.ResultsFive different subtypes of PvMSP-3β were identified based on single nucleotide polymorphisms (SNPs), insertions, and deletions. Although a high level of sequence diversity was observed in the PvMSP-3β gene, the coiled-coil tertiary structure of the PvMSP-3β protein was well conserved in all of the sequences. The PvMSP-3β of Korean isolates is under natural selection. DNA polymerase slippage and intragenic recombination likely contributed to PvMSP-3β diversity in Korean P. vivax isolates.ConclusionsThe PvMSP-3β of Korean P. vivax isolates displayed polymorphisms, with SNPs, insertions and deletions scattered throughout of the gene. These results of parasite heterogeneity are relevant to the development of a PvMSP-3β based vaccine against P. vivax and the implementation of malaria control programmes in Korea.

【 授权许可】

Unknown   
© Kang et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.