Brazilian Plasmodium falciparum isolates: investigation of candidate polymorphisms for artemisinin resistance before introduction of artemisinin-based combination therapy

Malaria Journal

Research

José M de Souza¹  Philip J Rosenthal²  Yonne FC Melo³  Fátima Santos⁴  Bianca E Gama⁵  Natália K Almeida de Oliveira⁵  Maria de Fátima Ferreira-da-Cruz⁵  Cláudio T Daniel-Ribeiro⁵  Leonardo JM de Carvalho⁶ 

[1] Ambulatory and Laboratory of Malaria Clinical Assays, Secretariat of Health Vigilance, Instituto Evandro Chagas, Belém (PA), Brazil;Department of Medicine, San Francisco General Hospital, University of California, San Francisco, USA;Fundação de Medicina Tropical do Amazonas, Manaus (AM), Brazil;Laboratory of Entomology, LACEN, Porto Velho (RO), Brazil;Laboratory of Malaria Research, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro (RJ), Brazil;Laboratory of Malaria Research, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro (RJ), Brazil;La Jolla Bioengineering Institute, California, USA;

关键词: Malaria;    Artesunate;    Artemether;    Malaria National Control Program;    Amodiaquine;   

DOI  :  10.1186/1475-2875-9-355

 received in 2010-10-20, accepted in 2010-12-08,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundThis study was performed to better understand the genetic diversity of known polymorphisms in pfatpase6 and pfmdr1 genes before the introduction of ACT in Brazil, in order to get a genotypic snapshot of Plasmodium falciparum parasites that may be used as baseline reference for future studies.MethodsParasites from P. falciparum samples collected in 2002, 2004 and 2006-2007 were genotyped using PCR and DNA sequencing at codons 86, 130, 184, 1034, 1042, 1109 and 1246 for pfmdr1 gene, and 243, 263, 402, 431, 623, 630, 639, 683, 716, 776, 769 and 771 for pfatpase6 gene.ResultsA pfmdr1 haplotype NEF/CDVY was found in 97% of the samples. In the case of pfatpase6, four haplotypes, wild-type (37%), 630 S (35%), 402 V (5%) and double-mutant 630 S + 402 V (23%), were detected.ConclusionAlthough some polymorphism in pfmdr1 and pfatpase6 were verified, no reported haplotypes in both genes that may mediate altered response to ACT was detected before the introduction of this therapy in Brazil. Thus, the haplotypes herein described can be very useful as a baseline reference of P. falciparum populations without ACT drug pressure.

【 授权许可】

Unknown   
© Gama et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.