期刊论文详细信息
Malaria Journal
Different mutation patterns of Plasmodium falciparum among patients in Jimma University Hospital, Ethiopia
Research
Michael Hölscher1  Nicole Berens-Riha1  Thomas Löscher1  Isabel Barreto Miranda1  Teferi Eshetu2  Sintayehu Fekadu3  Robert Gürkov4  Zelalem Tadesse5 
[1] Department of Infectious Diseases and Tropical Medicine, Ludwig Maximilians University, Leopoldstrasse 5, 80802, München, Germany;Department of Infectious Diseases and Tropical Medicine, Ludwig Maximilians University, Leopoldstrasse 5, 80802, München, Germany;Department of Microbiology, Parasitology and Immunology, Jimma University, Jimma, Ethiopia;Department of Internal Medicine, Jimma University, Jimma, Ethiopia;Department of Otorhinolaryngology, Head and Neck Surgery, Ludwig Maximilians University, Munich, Germany;Department of Paediatrics, Jimma University, Jimma, Ethiopia;
关键词: Malaria;    Artesunate;    Artemether;    Atovaquone;    Proguanil;   
DOI  :  10.1186/1475-2875-9-226
 received in 2010-06-07, accepted in 2010-08-07,  发布年份 2010
来源: Springer
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【 摘 要 】

BackgroundThe emergence of drug resistance is a major problem in malaria control. Combination of molecular genotyping and characterization of mutations or single nucleotide polymorphisms (SNPs) correlated with drug resistance can provide information for subsequent surveillance of existing and developing drug resistance patterns. The introduction of artemether/lumefantrine (AL) as first-line treatment, never used before in Ethiopia, allowed the collection of baseline data of molecular polymorphisms before a selection due to AL could occur.Method97 patients with uncomplicated falciparum malaria were recruited from April to June 2006 and treated with either AL, quinine (Q) or atovaquone/proguanil (AP) in Jimma University Hospital, Ethiopia. Mutations or SNPs associated with resistance to these drugs were analysed by RFLP (pfdhfr, pfmdr1) and sequencing of the target genes (pfcytb, pfserca ).ResultsSNPs previously reported to be associated with resistance to the study drugs were identified in recrudescent and treatment sensitive isolates. A total of seven recrudescences were obtained. The pfmdr1 N86Y mutation was found in 84.5% of isolates. The triple mutation 51I,59R,108N of the pfdhfr gene occured in high frequency (83.3%) but no pfcytb mutation was detected. Sequencing showed a variety of previously described and new mutations in the pfserca gene.ConclusionThe prevalence of mutations was in accordance with the expected patterns considering recent drug regimens. The broad introduction of AL and the cessation of former drug regimens might probably change the current distribution of polymorphisms, possibly leading to decreased sensitivity to AL in future. Continuous surveillance of molecular patterns in this region is, therefore, recommended.

【 授权许可】

Unknown   
© Eshetu et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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