Comparison of freezing tolerance, compatible solutes and polyamines in geographically diverse collections of Thellungiella sp. and Arabidopsis thaliana accessions

BMC Plant Biology

Research Article

Alexei Babakov¹  Bert de Boer²  Yang Ping Lee³  Ellen Zuther³  Dirk K Hincha³ 

[1] All-Russia Research Institute of Agricultural Biotechnology RAAS, Timiryazevskaya St. 42, 127550, Moscow, Russia;Department of Structural Biology, Vrije Universiteit Amsterdam, De Boelelaan 1085-1087, 1081, Amsterdam, HV, The Netherlands;Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, D-14476, Potsdam, Germany;

关键词: Arabidopsis thaliana;    Cold acclimation;    Compatible solutes;    Freezing tolerance;    Natural variation;    Polyamines;    Thellungiella salsuginea;   

DOI  :  10.1186/1471-2229-12-131

 received in 2012-04-24, accepted in 2012-07-13,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundThellungiella has been proposed as an extremophile alternative to Arabidopsis to investigate environmental stress tolerance. However, Arabidopsis accessions show large natural variation in their freezing tolerance and here the tolerance ranges of collections of accessions in the two species were compared.ResultsLeaf freezing tolerance of 16 Thellungiella accessions was assessed with an electrolyte leakage assay before and after 14 days of cold acclimation at 4°C. Soluble sugars (glucose, fructose, sucrose, raffinose) and free polyamines (putrescine, spermidine, spermine) were quantified by HPLC, proline photometrically. The ranges in nonacclimated freezing tolerance completely overlapped between Arabidopsis and Thellungiella. After cold acclimation, some Thellungiella accessions were more freezing tolerant than any Arabidopsis accessions. Acclimated freezing tolerance was correlated with sucrose levels in both species, but raffinose accumulation was lower in Thellungiella and only correlated with freezing tolerance in Arabidopsis. The reverse was true for leaf proline contents. Polyamine levels were generally similar between the species. Only spermine content was higher in nonacclimated Thellungiella plants, but decreased during acclimation and was negatively correlated with freezing tolerance.ConclusionThellungiella is not an extremophile with regard to freezing tolerance, but some accessions significantly expand the range present in Arabidopsis. The metabolite data indicate different metabolic adaptation strategies between the species.

【 授权许可】

Unknown   
© Lee et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.