Molecular and structural analyses of a novel temperature stress‐induced lipocalin from wheat and Arabidopsis

FEBS Letters

Sarhan, Fathey¹  Frenette Charron, Jean-Benoit¹  Badawi, Mohamed¹  Breton, Ghislain¹ 

[1] Département des Sciences Biologiques, Université du Québec à Montréal, P.O. Box 8888, Succ. “Centre-Ville”, Montréal, QC, Canada H3C 3P8

关键词: Wheat;    Cold acclimation;    Heat-shock;    Apolipoprotein D;    Lipocalin;    Freezing tolerance;    ABA;    abscisic acid;    At;    Arabidopsis thaliana;    ApoD;    apolipoprotein D;    Blc;    bacterial lipocalin;    GPI;    glycosylphosphatidylinositol;    SCR;    structurally conserved region;    SSC;    saline sodium citrate;    Ta;    Triticum aestivum L;    TIL;    temperature-induced lipocalin;   

DOI  :  10.1016/S0014-5793(02)02606-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two cDNAs corresponding to a novel lipocalin were identified from wheat and Arabidopsis. The two cDNAs designated Tatil for Triticum aestivum L. temperature-induced lipocalin and Attil for Arabidopsis thaliana temperature-induced lipocalin encode polypeptides of 190 and 186 amino acids respectively. Structure analyses indicated the presence of the three structurally conserved regions that characterize lipocalins. Sequence analyses revealed that this novel class of plant lipocalin shares homology with three evolutionarily related lipocalins: the mammalian apolipoprotein D (ApoD), the bacterial lipocalin and the insect Lazarillo. The comparison of the putative tertiary structures of both the human ApoD and the wheat TaTIL suggest that the two proteins differ in membrane attachment and ligand interaction. Northern analyses demonstrated that Tatil and Attil transcripts are upregulated during cold acclimation and heat-shock treatment. The putative functions of this novel class of plant lipocalins during temperature stresses are discussed.

【 授权许可】

Unknown