Decreased T helper 17 cells in tuberculosis is associated with increased percentages of programmed death ligand 1, T helper 2 and regulatory T cells

Respiratory Research

Research

Jann-Yuan Wang¹  Chong-Jen Yu¹  Li-Na Lee²  Hsin-Chih Lai³  Bor-Luen Chiang⁴  Chin-Chung Shu⁵  Ming-Fang Wu⁶ 

[1] Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan;Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan;Department of Laboratory Medicine, National Taiwan University Hospital, Taipei, Taiwan;Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Tao-Yuan, Taiwan;Department of Pediatrics, National Taiwan University Hospital, Taipei, Taiwan;Department of Medical Research, National Taiwan University Hospital, #7, Chung-Shan South Road, 100, Taipei, Taiwan;Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan;Department of Traumatology, National Taiwan University Hospital, Taipei, Taiwan;Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan;Graduate Institute of Toxicology, College of Medicine, National Taiwan University, Taipei, Taiwan;

关键词: Programmed cell death 1;    Cytotoxic T-lymphocyte-associated protein 4;    Tuberculosis;    Th17 cells;    Regulatory T cells;    Th2 cells;   

DOI  :  10.1186/s12931-017-0580-3

 received in 2017-01-10, accepted in 2017-05-09,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundTuberculosis (TB) is one of the most common infectious diseases worldwide. During active tuberculosis, T helper (Th) 17 cells are decreased, however the association with inhibitory immune regulation is unclear.MethodsWe enrolled 27 patients with TB and 20 age- and sex-matched controls and studies their lymphocyte status. Peripheral blood lymphocytes were isolated and programmed death-1 (PD-1) and programmed death ligand 1 (PD-L1) were measured on Th17 cells by using flow cytometry after the cells were stimulated with phorbol 12-myristate 13-acetate and ionomycin for 6 h. In addition, Th2 and regulatory T cells were measured and analyzed.ResultsThe TB group had lower levels of Th17 cells but higher levels of Th2 and Treg cells than the controls. In Th17 cells, the percentage of PD-L1 was higher in the TB group than that in the controls. In Th2 and Treg cells, the percentage of cytotoxic T-lymphocyte associated protein 4 (CTLA-4) was lower in the TB group and PD-1 was higher in Treg cells in the TB group. In the patients with extra-pulmonary TB, levels of Th1, Th2 and T17 cells were lower than those with pulmonary TB. The percentage of PD-1 on Th1 lymphocytes positively correlated with radiographic score.ConclusionsLower level of Th17 in TB patients may be associated with increased percentage of PD-L1 and increasing levels of Th2 and Treg cells which influenced by CTLA-4.

【 授权许可】

CC BY   
© The Author(s). 2017