Action of nicotinic acid on the reversion of hypoxic-inflammatory link on 3T3-L1 adipocytes

Lipids in Health and Disease

Research

Renata Nakamichi¹  Beata Marie Redublo Quinto¹  Sylvia Madeira de Vergueiro Lobo¹  Vivian Regina Tristão¹  Erika Prates Miranda¹  Marcelo Costa Batista²  Maria Aparecida Dalboni³ 

[1] Nephrology Division, Department of Medicine, Universidade Federal de São Paulo, Rua Pedro de Toledo 781, Vila Clementino, São Paulo, Brazil;Nephrology Division, Department of Medicine, Universidade Federal de São Paulo, Rua Pedro de Toledo 781, Vila Clementino, São Paulo, Brazil;Dialysis Unit, Intensive Care Center, Hospital Israelita Albert Einstein, São Paulo, Brazil;Division of Nephrology, Tufts University School of Medicine, Boston, USA;Nephrology Division, Department of Medicine, Universidade Federal de São Paulo, Rua Pedro de Toledo 781, Vila Clementino, São Paulo, Brazil;Universidade Nove de Julho, São Paulo, Brazil;

关键词: Hypoxia;    Inflammation;    Obesity;    Adipocyte;    Nicotinic acid;   

DOI  :  10.1186/s12944-016-0260-1

 received in 2015-11-17, accepted in 2016-05-03,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundHypoxia resulting from adipocyte expansion is considered the basis of the inflammatory milieu observed in Metabolic Syndrome. Nicotinic acid can act on adipocytes interfering on the inflammatory response. In this study, we investigated the role of HIF-1 α (hypoxia-inducible factor -1 alpha) in the inflammatory process induced by hypoxia. The effect of nicotinic acid on the PPARs (peroxisome proliferator-activated receptors) expression during the inflammatory response was assessed over its action under HIF-1 α in 3T3-L1 adipocytes submitted to hypoxia.Methods3T3-L1 adipocytes were pre-treated with nicotinic acid and incubated under hypoxic conditions. The level of adipokines and HIF-1 α were quantified using immunoassays. Adipokine expression was measured using real-time PCR, whereas PPARs and HIF-1 α expression were analyzed by western blot. The statistical significance of the differences between variables studied was determined by analysis of variance (ANOVA) complemented by Bonferroni’s test.ResultsThe results demonstrated an increase in leptin and PAI-1 (plasminogen activator inhibitor-1) expression, while adiponectin production decreased under hypoxia. In parallel, induction with hypoxia enhanced HIF-1 α expression, despite causing reduced expression of PPAR α and PPAR γ. However, nicotinic acid reversed adipokine modulation under hypoxic conditions, leading to decreased HIF-1 α expression and increased PPARs expression.ConclusionsOur findings suggest that nicotinic acid blunt the inflammatory response resulting from hypoxia by the reduction of HIF-1 α expression and concomitant increase of PPARs α and γ expression in 3T3-L1 adipocytes.

【 授权许可】

CC BY   
© Nakamichi et al. 2016