Endoplasmic reticulum stress regulates inflammation in adipocyte of obese rats via toll-like receptors 4 signaling

Iranian Journal of Basic Medical Sciences

卷:21

Xiaohua Li¹ 

[1] Department of Endocrinology, Seventh People’s Hospital of Shanghai University of TCM , No. 358, Datong Road, Gaoqiao, Pudong New Area Shanghai, China;

关键词: Adipocyte;    Endoplasmic reticulum stress;    Inflammation;    Obesity;    Toll-like receptor 4;   

DOI  :  10.22038/ijbms.2018.27346.6674

来源: DOAJ

【 摘 要 】

Objective(s): To explore whether endoplasmic reticulum (ER) stress regulates inflammation in adipose tissue of obese rats via TLR4 signaling. Materials and Methods: Sprague Dawley rats were randomly divided into four groups, and body weight, food intake, and free fatty acids (FFA) were measured. Real-time PCR and Western blot were used to determine mRNA or protein expression of TLR4, TRAF6, IKKβ, TNF-α, IL-6, and GRP78. Immunohistochemistry was used to detect GRP78 protein expression. Results: The FFA levels in HFD, HFD+PBA, and HFD+VIPER groups were higher than that in the control group (P

【 授权许可】

Unknown