Secondhand smoke exposure toxicity accelerates age-related cardiac disease in old hamsters

BMC Cardiovascular Disorders

Research Article

Wei-Wen Kuo¹  Cheng-Hong Hsieh²  Yu-Lan Yeh³  Chia-Hua Kuo⁴  Jia-Ping Wu⁵  Chih-Yang Huang⁶  Chien-Chung Lin⁷  Tsung-Jung Ho⁸ 

[1] Department of Biological Science and Technology, China Medical University, Taichung, Taiwan;Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan;Department of Pathology, Changhua Christian Hospital, Changhua, Taiwan;Department of Medical Technology, Jen-Teh Junior College of Medicine, Nursing and Management, Miaoli, Taiwan;Department of Sports Sciences, University of Taipei, Taipei, Taiwan;Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan;Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan;Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan;School of Chinese Medicine, China Medical University, Taichung, Taiwan;Orthopedic Department, Armed Forces General Hospital, Taichung, Taiwan;School of Chinese Medicine, College of Chinese Medicine, China Medical University Beigang Hospital, Taichung, Taiwan;Chinese Medicine Department, China Medical University Beigang Hospital, Beigang, Taiwan;

关键词: Left ventricular hypertrophy;    Secondhand smoke exposure;    Aging;    Pro-inflammatory response;    Aged SHS exposure;   

DOI  :  10.1186/1471-2261-14-195

 received in 2014-09-08, accepted in 2014-12-11,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundAging is associated with physiological or pathological left ventricular hypertrophy (LVH) cardiac changes. Secondhand smoke (SHS) exposure is associated with pathological LVH. The action mechanism in cardiac concentric hypertrophy from SHS exposure is understood, but the transition contributed from SHS exposure is not. To determine whether exposure to SHS has an impact on age-induced LVH we examined young and old hamsters that underwent SHS exposure in a chamber for 30 mins.MethodsMorphological and histological studies were then conducted using hematoxylin and eosin (H&E) and Masson’s trichrome staining. Echocardiographic analysis was used to determine left ventricular wall thickness and function. LVH related protein expression levels were detected by western blot analysis.ResultsThe results showed that both young and aged hamsters exposed to SHS exhibited increased heart weights and left ventricular weights, left ventricular posterior wall thickness and intraventricular septum systolic and diastolic pressure also increased. However, left ventricular function systolic and diastolic pressure deteriorated. H&E and Masson’s trichrome staining results showed LV papillary muscles were ruptured, resulting in lower cardiac function at the myocardial level. LV muscle fiber arrangement was disordered and collagen accumulation occurred. Concentric LVH related protein molecular markers increased only in young hamsters exposed to SHS. However, this declined with hamster age. By contrast, eccentric LVH related proteins increased in aging hamsters exposed the SHS. Pro-inflammatory proteins, IL-6, TNF-α, JAK1, STAT3, and SIRTI expression increased in aging hamsters exposed to SHS.ConclusionsWe suggest that SHS exposure induces a pro-inflammatory response that results in concentric transition to aging eccentric LVH.

【 授权许可】

Unknown   
© Wu et al.; licensee BioMed Central. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.