Secondhand smoke exposure toxicity accelerates age-related cardiac disease in old hamsters

BMC Cardiovascular Disorders

Chih-Yang Huang²  Chia-Hua Kuo⁷  Chien-Chung Lin¹  Yu-Lan Yeh³  Wei-Wen Kuo⁵  Tsung-Jung Ho⁴  Cheng-Hong Hsieh²  Jia-Ping Wu⁶ 

[1] Orthopedic Department, Armed Forces General Hospital, Taichung, Taiwan;Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan;Department of Medical Technology, Jen-Teh Junior College of Medicine, Nursing and Management, Miaoli, Taiwan;Chinese Medicine Department, China Medical University Beigang Hospital, Beigang, Taiwan;Department of Biological Science and Technology, China Medical University, Taichung, Taiwan;Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan;Department of Sports Sciences, University of Taipei, Taipei, Taiwan

关键词: Aged SHS exposure;    Pro-inflammatory response;    Aging;    Secondhand smoke exposure;    Left ventricular hypertrophy;   

Others  :  1117927
DOI  :  10.1186/1471-2261-14-195

 received in 2014-09-08, accepted in 2014-12-11,  发布年份 2014

【 摘 要 】

Background

Aging is associated with physiological or pathological left ventricular hypertrophy (LVH) cardiac changes. Secondhand smoke (SHS) exposure is associated with pathological LVH. The action mechanism in cardiac concentric hypertrophy from SHS exposure is understood, but the transition contributed from SHS exposure is not. To determine whether exposure to SHS has an impact on age-induced LVH we examined young and old hamsters that underwent SHS exposure in a chamber for 30 mins.

Methods

Morphological and histological studies were then conducted using hematoxylin and eosin (H&E) and Masson’s trichrome staining. Echocardiographic analysis was used to determine left ventricular wall thickness and function. LVH related protein expression levels were detected by western blot analysis.

Results

The results showed that both young and aged hamsters exposed to SHS exhibited increased heart weights and left ventricular weights, left ventricular posterior wall thickness and intraventricular septum systolic and diastolic pressure also increased. However, left ventricular function systolic and diastolic pressure deteriorated. H&E and Masson’s trichrome staining results showed LV papillary muscles were ruptured, resulting in lower cardiac function at the myocardial level. LV muscle fiber arrangement was disordered and collagen accumulation occurred. Concentric LVH related protein molecular markers increased only in young hamsters exposed to SHS. However, this declined with hamster age. By contrast, eccentric LVH related proteins increased in aging hamsters exposed the SHS. Pro-inflammatory proteins, IL-6, TNF-α, JAK1, STAT3, and SIRTI expression increased in aging hamsters exposed to SHS.

Conclusions

We suggest that SHS exposure induces a pro-inflammatory response that results in concentric transition to aging eccentric LVH.

【 授权许可】

   
2014 Wu et al.; licensee BioMed Central.

【 图 表 】

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