Adhesion to and biofilm formation on IB3-1 bronchial cells by Stenotrophomonas maltophilia isolates from cystic fibrosis patients

BMC Microbiology

Research Article

Ersilia Fiscarelli¹  Vincenzo Savini²  Simone Guarnieri³  Mauro Nicoletti⁴  Valentina Crocetta⁵  Pamela Confalone⁵  Arianna Pompilio⁵  Giovanni Di Bonaventura⁵  Raffaele Piccolomini⁵  Andrea Petrucca⁶ 

[1] Clinical Microbiology Laboratory, Pediatric Hospital "Bambino Gesù", Rome, Italy;Clinical Microbiology and Virology, Department of Transfusion Medicine, "Spirito Santo" Hospital, Pescara, Italy;Department of Basic and Applied Medical Sciences, "G. D'Annunzio" University of Chieti-Pescara, Abruzzo, Italy;Department of Biomedical Sciences, "G. D'Annunzio" University of Chieti-Pescara, Abruzzo, Italy;Department of Biomedical Sciences, "G. D'Annunzio" University of Chieti-Pescara, Abruzzo, Italy;Center of Excellence for Aging, "G. D'Annunzio" University Foundation of Chieti-Pescara, Abruzzo, Italy;Department of Biomedical Sciences, "G. D'Annunzio" University of Chieti-Pescara, Abruzzo, Italy;Laboratory of Microbiology, "Sant'Andrea" Hospital, Rome, Italy;

关键词: Cystic Fibrosis;    Cystic Fibrosis Transmembrane Conductance Regulator;    Cystic Fibrosis Patient;    Abiotic Surface;    Cystic Fibrosis Lung Disease;   

DOI  :  10.1186/1471-2180-10-102

 received in 2009-09-30, accepted in 2010-04-07,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundStenotrophomonas maltophilia has recently gained considerable attention as an important emerging pathogen in cystic fibrosis (CF) patients. However, the role of this microorganism in the pathophysiology of CF lung disease remains largely unexplored. In the present study for the first time we assessed the ability of S. maltophilia CF isolates to adhere to and form biofilm in experimental infection experiments using the CF-derived bronchial epithelial IB3-1cell line. The role of flagella on the adhesiveness of S. maltophilia to IB3-1 cell monolayers was also assessed by using fliI mutant derivative strains.ResultsAll S. maltophilia CF isolates tested in the present study were able, although at different levels, to adhere to and form biofilm on IB3-1 cell monolayers. Scanning electron and confocal microscopy revealed S. maltophilia structures typical of biofilm formation on bronchial IB3-1 cells. The loss of flagella significantly (P < 0.001) decreased bacterial adhesiveness, if compared to that of their parental flagellated strains. S. maltophilia CF isolates were also able to invade IB3-1 cells, albeit at a very low level (internalization rate ranged from 0.01 to 4.94%). Pre-exposure of IB3-1 cells to P. aeruginosa PAO1 significantly increased S. maltophilia adhesiveness. Further, the presence of S. maltophilia negatively influenced P. aeruginosa PAO1 adhesiveness.ConclusionsThe main contribution of the present study is the finding that S. maltophilia is able to form biofilm on and invade CF-derived IB3-1 bronchial epithelial cells, thus posing a rationale for the persistence and the systemic spread of this opportunistic pathogen in CF patients. Experiments using in vivo models which more closely mimic CF pulmonary tissues will certainly be needed to validate the relevance of our results.

【 授权许可】

Unknown   
© Pompilio et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.