Comparison of culture and qPCR for the detection of Pseudomonas aeruginosa in not chronically infected cystic fibrosis patients

BMC Microbiology

Research Article

Kristine Desager¹  Sabine Van daele²  Frans De Baets²  Filomeen Haerynck²  Petra Schelstraete²  Hedwige Boboli³  Frédéric Pierart³  Christiane Knoop⁴  Georges Casimir⁴  Patrick Lebecque⁵  Elke De Wachter⁶  Anne Malfroot⁶  Guido Lopes dos Santos Santiago⁷  Mario Vaneechoutte⁷  Pieter Deschaght⁷  Leen Van Simaey⁷ 

[1] Cystic Fibrosis Centre Antwerp, University Hospital Antwerp, Antwerp, Belgium;Cystic Fibrosis Centre Ghent, Ghent University Hospital, Ghent, Belgium;Cystic Fibrosis Centre Liege, CHR La Citadelle and CHC Espérance, Liege, Belgium;Cystic Fibrosis Centre ULB, Hôpital Universitaire des Enfants Reine Fabiola, Erasme University Hospital, Brussels, Belgium;Cystic Fibrosis Centre, Cliniques St Luc, University of Louvain, Brussels, Belgium;Cystic Fibrosis Centre, Universitair Ziekenhuis Brussel, Brussels, Belgium;Laboratory for Bacteriology Research (LBR), Ghent University Hospital, Ghent University, Ghent, Belgium;

关键词: Cystic Fibrosis;    Cystic Fibrosis Transmembrane Conductance Regulator;    Cystic Fibrosis Patient;    Respiratory Sample;    Internal Amplification Control;   

DOI  :  10.1186/1471-2180-10-245

 received in 2010-04-29, accepted in 2010-09-24,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundPseudomonas aeruginosa is the major respiratory pathogen causing severe lung infections among CF patients, leading to high morbidity and mortality. Once infection is established, early antibiotic treatment is able to postpone the transition to chronic lung infection. In order to optimize the early detection, we compared the sensitivity of microbiological culture and quantitative PCR (qPCR) for the detection of P. aeruginosa in respiratory samples of not chronically infected CF patients.ResultsIn this national study, we followed CF patients during periods between 1 to 15 months. For a total of 852 samples, 729 (86%) remained P. aeruginosa negative by both culture and qPCR, whereas 89 samples (10%) were positive by both culture and qPCR.Twenty-six samples were negative by culture but positive by qPCR, and 10 samples were positive by culture but remained negative by qPCR. Five of the 26 patients with a culture negative, qPCR positive sample became later P. aeruginosa positive both by culture and qPCR.ConclusionBased on the results of this study, it can be concluded that qPCR may have a predictive value for impending P. aeruginosa infection for only a limited number of patients.

【 授权许可】

CC BY   
© Deschaght et al; licensee BioMed Central Ltd. 2010