Steering cell migration by alternating blebs and actin-rich protrusions

BMC Biology

Research Article

Weimiao Yu¹  Carl-Philipp Heisenberg²  Pawel Romanczuk³  Guillaume Salbreux⁴  Alba Diz-Muñoz⁵  Martin Bergert⁶  Ewa K. Paluch⁷  Kenzo Ivanovitch⁸ 

[1] Institute of Molecular and Cell Biology, A-STAR, 138673, Singapore, Singapore;Institute of Science and Technology Austria, 3400, Klosterneuburg, Austria;Max Planck Institute for the Physics of Complex Systems, 01187, Dresden, Germany;Department of Biology, Institute of Theoretical Biology, Humboldt University, 10115, Berlin, Germany;Max Planck Institute for the Physics of Complex Systems, 01187, Dresden, Germany;The Francis Crick Institute, Lincoln’s Inn Fields Laboratories, 44 Lincolns Inn Fields, WC2A 3LY, London, UK;Max Planck Institute of Molecular Cell Biology and Genetics, 01307, Dresden, Germany;International Institute of Molecular and Cell Biology, 02-109, Warsaw, Poland;Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, 69117, Heidelberg, Germany;Max Planck Institute of Molecular Cell Biology and Genetics, 01307, Dresden, Germany;International Institute of Molecular and Cell Biology, 02-109, Warsaw, Poland;Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, 69117, Heidelberg, Germany;Medical Research Council Laboratory for Molecular Cell Biology, University College London, WC1E 6BT, London, UK;Max Planck Institute of Molecular Cell Biology and Genetics, 01307, Dresden, Germany;International Institute of Molecular and Cell Biology, 02-109, Warsaw, Poland;Medical Research Council Laboratory for Molecular Cell Biology, University College London, WC1E 6BT, London, UK;Medical Research Council Laboratory for Molecular Cell Biology, University College London, WC1E 6BT, London, UK;Present address: Department of Cardiovascular Development and Repair, Centro Nacional de Investigaciones Cardiovasculares (CNIC), 28029, Madrid, Spain;

关键词: Cell migration;    Protrusion orientation;    Directionality;    Persistence;    Bleb;    Actin-rich protrusion;    Run and tumble;   

DOI  :  10.1186/s12915-016-0294-x

 received in 2016-06-20, accepted in 2016-08-08,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundHigh directional persistence is often assumed to enhance the efficiency of chemotactic migration. Yet, cells in vivo usually display meandering trajectories with relatively low directional persistence, and the control and function of directional persistence during cell migration in three-dimensional environments are poorly understood.ResultsHere, we use mesendoderm progenitors migrating during zebrafish gastrulation as a model system to investigate the control of directional persistence during migration in vivo. We show that progenitor cells alternate persistent run phases with tumble phases that result in cell reorientation. Runs are characterized by the formation of directed actin-rich protrusions and tumbles by enhanced blebbing. Increasing the proportion of actin-rich protrusions or blebs leads to longer or shorter run phases, respectively. Importantly, both reducing and increasing run phases result in larger spatial dispersion of the cells, indicative of reduced migration precision. A physical model quantitatively recapitulating the migratory behavior of mesendoderm progenitors indicates that the ratio of tumbling to run times, and thus the specific degree of directional persistence of migration, are critical for optimizing migration precision.ConclusionsTogether, our experiments and model provide mechanistic insight into the control of migration directionality for cells moving in three-dimensional environments that combine different protrusion types, whereby the proportion of blebs to actin-rich protrusions determines the directional persistence and precision of movement by regulating the ratio of tumbling to run times.

【 授权许可】

CC BY   
© Diz-Muñoz et al. 2016