The mechanism of dietary cholesterol effects on lipids metabolism in rats

Lipids in Health and Disease

Research

Bei Zhang¹  Yu-Ming Wang¹  Chang-Hu Xue¹  Zhao-Jie Li¹  Yong Xue¹  Jing-Feng Wang¹  Teruyoshi Yanagita² 

[1] College of Food Science and Engineering, Ocean University of China, Qingdao, China;Department of Applied Biological Sciences, Saga University, Saga, Japan;

关键词: Cholesterol Ester;    Dietary Cholesterol;    High Dietary Cholesterol;    Hepatic Lipid Metabolism;    Cholesterol Catabolism;   

DOI  :  10.1186/1476-511X-9-4

 received in 2009-12-09, accepted in 2010-01-14,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundCholesterol administration has been reported to influence hepatic lipid metabolism in rats. In the present study, the effect of dietary cholesterol on hepatic activity and mRNA expression of the enzymes involved in lipid metabolism were investigated. Fourteen male Wistar rats were randomly divided into 2 groups and fed 1% cholesterol or cholesterol free AIN76 diets for 4 weeks.ResultsThe serum triglyceride and high density lipoprotein cholesterol levels were significantly decreased but the total cholesterol and non high density lipoprotein cholesterol levels were significantly increased in the cholesterol-fed rats compared with the control rats. And the concentrations of the hepatic total cholesterol and triglyceride increased about 4-fold and 20-fold separately by dietary cholesterol. The activities of hepatic malic enzyme, glucose-6-phosphate dehydrogenase, fatty acid synthase, phosphatidate phophatase and carnitine palmitoyl transferase were depressed by the cholesterol feeding (40%, 70%, 50%, 15% and 25% respectively). The results of mRNA expression showed that fatty acid synthase, carnitine palmitoyl transferase 1, carnitine palmitoyl transferase 2, and HMG-CoA reductase were down-regulated (35%, 30%, 50% and 25% respectively) and acyl-CoA: cholesterol acyltransferase and cholesterol 7α-hydroxylase were up regulated (1.6 and 6.5 folds) in liver by the cholesterol administration.ConclusionsThe dietary cholesterol increased the triglyceride accumulation in liver, but did not stimulate the activity and the gene expression of hepatic enzymes related to triglyceride and fatty acid biosynthesis.

【 授权许可】

Unknown   
© Wang et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.