Putative virulence factors of Corynebacterium pseudotuberculosis FRC41: vaccine potential and protein expression

Microbial Cell Factories

Research

Vasco Azevedo¹  Natayme R. Tartaglia¹  Karina T. O. Santana-Jorge¹  Edgar L. Aguiar¹  Renata F. S. Souza¹  Túlio M. Santos²  Ricardo W. Portela³  Roberto Meyer³  Ricardo B. Mariutti⁴  Raphael J. Eberle⁴  Raghuvir K. Arni⁴ 

[1] Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antonio Carlos, 6627, Pampulha, 31270-901, Belo Horizonte, Brazil;Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antonio Carlos, 6627, Pampulha, 31270-901, Belo Horizonte, Brazil;Uniclon Biotecnologia, Belo Horizonte, MG, Brazil;Laboratório de Imunologia e Biologia Molecular, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, BA, Brazil;Multiuser Center for Biomolecular Innovation, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista “Júlio de Mesquita Filho”, São José Do Rio Preto, SP, Brazil;

关键词: Corynebacterium pseudotuberculosis;    Pathogenicity and virulence;    Vaccine potential;    Epitope prediction;    Protein expression;    Protein purification;   

DOI  :  10.1186/s12934-016-0479-6

 received in 2016-03-14, accepted in 2016-05-03,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundCorynebacterium pseudotuberculosis, a facultative intracellular bacterial pathogen, is the etiological agent of caseous lymphadenitis (CLA), an infectious disease that affects sheep and goats and it is responsible for significant economic losses. The disease is characterized mainly by bacteria-induced caseous necrosis in lymphatic glands. New vaccines are needed for reliable control and management of CLA. Thus, the putative virulence factors SpaC, SodC, NanH, and PknG from C. pseudotuberculosis FRC41 may represent new target proteins for vaccine development and pathogenicity studies.ResultsSpaC, PknG and NanH presented better vaccine potential than SodC after in silico analyses. A total of 136 B and T cell epitopes were predicted from the four putative virulence factors. A cluster analysis was performed to evaluate the redundancy degree among the sequences of the predicted epitopes; 57 clusters were formed, most of them (34) were single clusters. Two clusters from PknG and one from SpaC grouped epitopes for B and T-cell (MHC I and II). These epitopes can thus potentially stimulate a complete immune response (humoral and cellular) against C. pseudotuberculosis. Several other clusters, including two from NanH, grouped B-cell epitopes with either MHC I or II epitopes. The four target proteins were expressed in Escherichia coli. A purification protocol was developed for PknG expression.ConclusionsIn silico analyses show that the putative virulence factors SpaC, PknG and NanH present good potential for CLA vaccine development. Target proteins were successfully expressed in E. coli. A protocol for PknG purification is described.

【 授权许可】

CC BY   
© The Author(s). 2016