Equivalent susceptibility of Anopheles gambiae M and S molecular forms and Anopheles arabiensis to Plasmodium falciparum infection in Burkina Faso

Malaria Journal

Research

Wamdaogo M Guelbéogo¹  Soumanaba Zongo¹  Antoine Sanou¹  N’Falé Sagnon¹  Alphonse Traoré¹  Awa Gnémé²  Michelle M Riehle³  Karin Eiglmeier⁴  Kenneth D Vernick⁵  Gustave B Kabré⁶ 

[1] Centre National de Recherche et de Formation sur le Paludisme, Ouagadougou, Burkina Faso;Centre National de Recherche et de Formation sur le Paludisme, Ouagadougou, Burkina Faso;Université de Ouagadougou, Ouagadougou, Burkina Faso;Department of Microbiology, University of Minnesota, Minneapolis, Minnesota, USA;Department of Parasitology and Mycology, Unit of Genetics and Genomics of Insect Vectors, Institut Pasteur, Paris, France;CNRS Unit of Hosts, Vectors and Pathogens (URA3012), Paris, France;Department of Parasitology and Mycology, Unit of Genetics and Genomics of Insect Vectors, Institut Pasteur, Paris, France;CNRS Unit of Hosts, Vectors and Pathogens (URA3012), Paris, France;Department of Microbiology, University of Minnesota, Minneapolis, Minnesota, USA;Université de Ouagadougou, Ouagadougou, Burkina Faso;

关键词: Anopheles gambiae;    Anopheles arabiensis;    Plasmodium falciparum;    Malaria vector;    Genetic susceptibility;    Molecular form;    Infection prevalence;    Infection intensity;   

DOI  :  10.1186/1475-2875-12-204

 received in 2013-04-15, accepted in 2013-06-11,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundThe Anopheles gambiae sensu lato (s.l.) species complex in Burkina Faso consists of Anopheles arabiensis, and molecular forms M and S of Anopheles gambiae sensu stricto (s.s.). Previous studies comparing the M and S forms for level of infection with Plasmodium falciparum have yielded conflicting results.MethodsMosquito larvae were sampled from natural pools, reared to adulthood under controlled conditions, and challenged with natural P. falciparum by experimental feeding with blood from gametocyte carriers. Oocyst infection prevalence and intensity was determined one week after infection. DNA from carcasses was genotyped to identify species and molecular form.ResultsIn total, 7,400 adult mosquitoes grown from wild-caught larvae were challenged with gametocytes in 29 experimental infections spanning four transmission seasons. The overall infection prevalence averaged 40.7% for A. gambiae M form, 41.4% for A. gambiae S form, and 40.1% for A. arabiensis. There was no significant difference in infection prevalence or intensity between the three population groups. Notably, infection experiments in which the population groups were challenged in parallel on the same infective blood displayed less infection difference between population groups, while infections with less balanced composition of population groups had lower statistical power and displayed apparent differences that fluctuated more often from the null average.ConclusionThe study clearly establishes that, at the study site in Burkina Faso, there is no difference in genetic susceptibility to P. falciparum infection between three sympatric population groups of the A. gambiae s.l. complex. Feeding the mosquito groups on the same infective blood meal greatly increases statistical power. Conversely, comparison of the different mosquito groups between, rather than within, infections yields larger apparent difference between mosquito groups, resulting from lower statistical power and greater noise, and could lead to false-positive results. In making infection comparisons between population groups, it is more accurate to compare the different groups after feeding simultaneously upon the same infective blood.

【 授权许可】

Unknown   
© Gnémé et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.